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SGPL1 stimuates VPS39 recruitment to the mitochondria in MICU1deficient cells

ABSTRACT: SGPL1 stimuates VPS39 recruitment to the mitochondria in MICU1deficient cells

PROVIDER: PRJNA809669 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18112662.fastq.gz	Fastqsanger.gz
	SRR18112663.fastq.gz	Fastqsanger.gz
	SRR18112664.fastq.gz	Fastqsanger.gz
	SRR18112665.fastq.gz	Fastqsanger.gz
	SRR18112666.fastq.gz	Fastqsanger.gz

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Project description:ObjectiveMitochondrial "retrograde" signaling may stimulate organelle biogenesis as a compensatory adaptation to aberrant activity of the oxidative phosphorylation (OXPHOS) system. To maintain energy-consuming processes in OXPHOS deficient cells, alternative metabolic pathways are functionally coupled to the degradation, recycling and redistribution of biomolecules across distinct intracellular compartments. While transcriptional regulation of mitochondrial network expansion has been the focus of many studies, the molecular mechanisms promoting mitochondrial maintenance in energy-deprived cells remain poorly investigated.MethodsWe performed transcriptomics, quantitative proteomics and lifespan assays to identify pathways that are mechanistically linked to mitochondrial network expansion and homeostasis in Caenorhabditis elegans lacking the mitochondrial calcium uptake protein 1 (MICU-1/MICU1). To support our findings, we carried out biochemical and image analyses in mammalian cells and mouse-derived tissues.ResultsWe report that micu-1(null) mutations impair the OXPHOS system and promote C. elegans longevity through a transcriptional program that is independent of the mitochondrial calcium uniporter MCU-1/MCU and the essential MCU regulator EMRE-1/EMRE. We identify sphingosine phosphate lyase SPL-1/SGPL1 and the ATFS-1-target HOPS complex subunit VPS-39/VPS39 as critical lifespan modulators of micu-1(null) mutant animals. Cross-species investigation indicates that SGPL1 upregulation stimulates VPS39 recruitment to the mitochondria, thereby enhancing mitochondria-lysosome contacts. Consistently, VPS39 downregulation compromises mitochondrial network maintenance and basal autophagic flux in MICU1 deficient cells. In mouse-derived muscles, we show that VPS39 recruitment to the mitochondria may represent a common signature associated with altered OXPHOS system.ConclusionsOur findings reveal a previously unrecognized SGPL1/VPS39 axis that stimulates intracellular organelle interactions and sustains autophagy and mitochondrial homeostasis in OXPHOS deficient cells.

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SGPL1 stimuates VPS39 recruitment to the mitochondria in MICU1deficient cells

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