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Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (mRNA)

ABSTRACT: Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (mRNA)

PROVIDER: PRJNA812576 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18214054.fastq.gz	Fastqsanger.gz
	SRR18214055.fastq.gz	Fastqsanger.gz
	SRR18214056.fastq.gz	Fastqsanger.gz
	SRR18214057.fastq.gz	Fastqsanger.gz
	SRR18214058.fastq.gz	Fastqsanger.gz

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Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts

Project description:Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts

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Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (miRNA)

Project description:Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (miRNA)

| PRJNA812577 | ENA

Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (mRNA)

Project description:The current study is aimed on determine the functional impact of microRNA-378a-3p and microRNA-146b on embryo development. For such purpose, we supplemented miR-378/miR-146b mimics or inhibitors to the culture medium containing presumed zygotes at 1 dpi and cultured them until day 8, thus allowing miR-378/miR-146b mimics or inhibitors to influence further embryo development and quality. Further on, to gain more in-depth molecular insights, we performed transcriptome profiling of blastocysts cultured in the presence of miR-378/miR-146b mimics or inhibitors.

2022-03-10 | GSE197876 | GEO

Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts.

Project description:SignificanceHatching from the zona pellucida is a prerequisite for embryo implantation and is less likely to occur in vitro for reasons unknown. Extracellular vesicles (EVs) are secreted by the embryo into the culture medium. Yet the role that embryonic EVs and their cargo microRNAs (miRNAs) play in blastocyst hatching has not been elucidated, partially due to the difficulties of isolating them from low amounts of culture medium. Here, we optimized EV-miRNA isolation from medium conditioned by individually cultured bovine embryos and subsequently showed that miR-378a-3p, which was up-regulated in EVs secreted by blastocysts, plays a crucial role in promoting blastocyst hatching. This demonstrates the regulatory effect of miR-378-3p on hatching, which is an established embryo quality parameter linked with implantation.

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Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-03-10 | GSE197878 | GEO

Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (miRNA)

Project description:The current study is focused on Isolating EVs and their cargo content (miRNAs) from culture medium conditioned by individual embryos that can differentiate between the blastocyst and non-blastocyst embryos. We were able to isolate EVs from both blastocyst and non-blastocyst group with size exclusion chromatography (Izon’s™ qEV single column) and characterize them with Western blotting, nanoparticle tracking analysis, and Transmission electron microscopy. Further, we could isolate total RNA (including small RNAs) from the blastocyst and non-blastocyst EVs for miRNA sequencing.

2022-03-10 | GSE197875 | GEO

Edited miR-378a-3p target genes

Project description:Wild-type (WT) miR-378a-3p or edited miR-378a-3p were expressed in SB2 KD-ADAR1 cells to identify the genes regulated by edited miR-378a-3p vs WT miR-378a-3p. PARVA was one of the genes identified to be regulated by edited miR-378a-3p. We demonstrate that this regulation of PARVA is lost in highly metastatic melanoma cells. Microarray analysis was used to evaluate the robustness and reproducibility of the method used to generate the ex vivo tumor tissue model and confirm its ability to recapitulate the essential features of the original tumor.

2018-02-22 | GSE107088 | GEO

Gene expression profile changes during blastocysts hatching of embryos

Project description:Gene expression profile changes during blastocysts hatching of embryos

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MiR-378a-3p is critical for Burkitt lymphoma cell growth

Project description:To gain further knowledge on the role of microRNAs (miRNAs) in the pathogenesis of Burkitt lymphoma (BL), we performed small RNA sequencing in BL cell lines and normal germinal center B (GC-B) cells. This revealed 26 miRNAs with significantly different expression levels. Altered levels were validated by qRT-PCR for six of eight selected miRNAs. For five miRNAs, the differential expression pattern was confirmed in BL primary tissues compared to GC-B cells. Inhibition of miR-378a-3p reduced the growth of multiple BL cell lines. RNA immunoprecipitation of Argonaute 2 followed by microarray analysis (Ago2-RIP-Chip) upon inhibition and ectopic overexpression of miR-378a-3p revealed 63 and 20 putative miR-378a-3p targets, respectively. These included 28 genes with a putative miR-378a-3p binding site. Effective targeting by miR-378a-3p was confirmed by luciferase reporter assays for four out of eight selected genes: MNT, FOXP1, IRAK4, and lncRNA JPX, all of which have been implicated in proliferation and cancer. In summary, our study identified several differentially expressed miRNAs in BL. We identified miR-378a-3p as a miRNA with an oncogenic role in BL and revealed 4 novel miR-378a-3p target genes, i.e. MNT, FOXP1, IRAK4, and JPX.

2020-06-30 | GSE141691 | GEO

Edited miR-378a-3p target genes

Project description:Edited miR-378a-3p target genes

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