Project description:The goal of this study was to investigate the roles of Butyrophilin Subfamily 1 Member A1 (BTN1A1) in terms of the signaling pathway after overexpression of BTN1A1A1 gene. To this end, we conducted NanoString analysis. From NanoString analysis with BTN1A1-overexpressing PC3 cells, we concluded that BTN1A1 play a pivotal role in the regulation of several signaling pathway including Jak-STAT pathway that is involved in processes such as immunity, cell division, cell death and tumor formation.
Project description:HT-29 human colorectal cancer cells were treated in vitro with Vehicle control (Ctrl), MV-GFP (GFP), Triptolide (TRP), or MV-GFP + TRP (Combo). Three biological replicates were performed per treatment. RNA was extracted using NanoString protocols and analyzed with the nCounter Human Tumor Signaling 360 panel. RCC files for each replicate are provided as raw data. Data were processed using nSolver 4.0 Advanced Analysis module with standard normalization and Benjamini–Hochberg correction.