Project description:Pistacia chinensis Bunge is known as dioecious, but we have found wild monoecious individuals. In order to screen the candidate genes which may influence the sex expression or floral phenotypic differences of P. chinensis, the inflorescence buds for different sex types associated with the sex differentiation were selected and tested for small RNA sequencing. Sex-specific differentially expressed small RNA were discovered, combined with real-time PCR data, the regulation patterns of various sex types were first revealed. Our study represents the first detailed analysis of small RNA sequencing, providing more clues for understanding the mechanism of sex determination on P. chinensis.
Project description:To characterize the expression of 58219 genes annotated from garlic genome, the transcriptomes of seven tissues (including garlic sprouts, shoots, bulbs, flowers, roots, pesudostems, and leaves) were sequenced, and finally identified 45,750 genes expressed in various tissues investigated. In addition, the bulbs-transcriptomes in the eight stages of bulbs-development were sequenced, resulting in 6,234 genes isentified to show dynamic expression changes in the bulbs-developmental process.
Project description:We determined the alkaloid content in Lycoris longituba, Lycoris incarnata, and Lycoris sprengeri, and performed a SWATH-MS-based quantitative approach to detect proteome changes in the three Lycoris.
Project description:This study aimed to identify the mode of action of Schisandra chinensis water extracts (SCW) and Schisandra chinensis ethanol extracts (SCE) in SW1783 cell line.
Project description:Cholestatic liver disease (CLD) is characterized by the dysfunction of bile synthesis and excretion, leading to progressive liver injury or fibrosis, but lacks effective treatment drugs. Lycorine, an alkaloid isolated from Lycoris bulbs, possesses antiviral, anti-inflammatory, analgesic, cardioprotective activities. However, there has been no report concerning its effects on CLD so far. We evaluated the hepatoprotective effect of lycorine on intrahepatic cholestasis mouse models induced by α-naphthylisothiocyanate (ANIT) or after multidrug resistance protein 2 knockout (Mdr2-/-). RNA-Sequencing (RNA-Seq), cellular thermal shift assay (CETSA) and mass spectrometry analysis were used to investigate the regulated molecular mechanism of lycorine. Lycorine alleviated intrahepatic cholestasis by decreasing the expression of activating transcription factor 3 (ATF3), and inhibiting the nuclear accumulation of unphosphorylated ATF3, a transcription repression factor of the bile salt export pump (BSEP) responsible for bile acid (BA) secretion. Additionally, lycorine bound with transketolase (TKT) protein directly and promoted its ubiquitination degradation, disrupting the interaction between TKT and ATF3, resulting in the increased interaction between Rho-associated protein kinase 1(ROCK1) and ATF3 and subsequently BSEP transcription repression. Conclusively, our results shed light on the protective effects and mechanisms of lycorine against CLD, which pave way for the design of novel clinical therapeutic strategies.
Project description:Rhodobacter sphaeroides 2.4.1 illuminated with either lumiline bulbs at 10W/m2 or tungsten bulbs at 100W/m2. Sparged with 95%N/5%CO2. Keywords: other