Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Time course expression pattern during neural crest cells (NCCs) differentiation from iPSCs

ABSTRACT: Time course expression pattern during neural crest cells (NCCs) differentiation from iPSCs

PROVIDER: PRJNA848840 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19643028.fastq.gz	Fastqsanger.gz
	SRR19643029.fastq.gz	Fastqsanger.gz
	SRR19643030.fastq.gz	Fastqsanger.gz
	SRR19643031.fastq.gz	Fastqsanger.gz
	SRR19643032.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Time course expression pattern during neural crest cells (NCCs) differentiation from iPSCs

Project description:To clarify the changes in cell properties during the differentiation process from iPSCs into NCCs, we sampled each cell during the differentiation process and compared gene expression patterns.

2022-06-18 | GSE206048 | GEO

Generation of mesenchymal stem/stromal cells (MSCs) from human iPSCs via neural crest cells (NCCs)

Project description:To clarify the changes in cell properties during the differentiation process from iPSCs into MSC through NCCs, we sampled each cell during the differentiation process and compared gene expression patterns.

2022-06-19 | GSE206128 | GEO

Generation of mesenchymal stem/stromal cells (MSCs) from human iPSCs via neural crest cells (NCCs)

Project description:Generation of mesenchymal stem/stromal cells (MSCs) from human iPSCs via neural crest cells (NCCs)

| PRJNA849255 | ENA

Immunological properties of neural crest cells derived from human induced pluripotent stem cells (resting state)

Project description:Collecting primary neural crest cells (NCCs) of a sufficient quantity for some experiments is difficult, because NCCs are embryonic transient tissue and basically they do not proliferate. We successfully induced NCCs from human induced pluripotent stem cells (iPSCs) according to the previously described method with some modifications. iPSCs-derived NCCs (iPSC-NCCs) are reported to have potential for wound healing and expected to be used clinically in the future. However, nobody reported the immunological properties of iPSC-NCCs. It is important to evaluate the immunological properties of iPSC-NCCs before clinical use. We examined gene expression patterns between iPSCs and iPSC-NCCs.

2018-12-01 | GSE109136 | GEO

CHARGE syndrome modeling using patient-derived iPSC reveals defective migration of neural crest cells harboring CHD7 mutations

Project description:CHARGE syndrome is caused by heterozygous mutations in a chromatin remodeler CHD7 and characterized by a set of malformations historically postulated to arise from defects in the neural crest formation during embryogenesis. To better delineate neural crest defects in CHARGE syndrome, we generated induced pluripotent stem cells (iPSCs) from two patients with typical syndrome manifestations, and characterized neural crest cells differentiated in vitro from these iPSCs (iPSC-NCCs). We found that expression of genes associated with cell migration was altered in CHARGE iPSC-NCCs as compared to control iPSC-NCCs. Consistently, CHARGE iPSC-NCCs showed defective delamination, migration and motility in vitro, and their transplantation in ovo revealed overall defective migratory activity in the chick embryo. Altogether, our results support the historical inference that CHARGE syndrome patients have defects in neural crest migration and provide the first successful application of patient-derived iPSCs in modeling craniofacial disorders.

2017-10-23 | GSE86212 | GEO

Immunological properties of neural crest cells derived from human induced pluripotent stem cells (normal vs inflammed condition)

Project description:Collecting primary neural crest cells (NCCs) of a sufficient quantity for some experiments is difficult, because NCCs are embryonic transient tissue and basically they do not proliferate. We successfully induced NCCs from human induced pluripotent stem cells (iPSCs) according to the previously described method with some modifications. iPSCs-derived NCCs (iPSC-NCCs) are reported to have potential for wound healing and expected to be used clinically in the future. However, nobody reported the immunological properties of iPSC-NCCs. It is important to evaluate the immunological properties of iPSC-NCCs before clinical use. In our previous study, we found that iPSC-NCCs have immunosuppressive properties in resting state. In the next step, we examined gene expression patterns between iPSC-NCCs (normal condition) and iPSC-NCCs (inflamed condition).

2018-12-01 | GSE116110 | GEO

Time course gene expression pattern during neural crest cell (NCC) differentiation from induced pluripotent stem cells (iPSCs) in three-dimensional (3D) conditions

Project description:Time course gene expression pattern during neural crest cell (NCC) differentiation from induced pluripotent stem cells (iPSCs) in three-dimensional (3D) conditions

| PRJNA1088147 | ENA

Expression data from neural crest cells and neural crest cell-derived MSCs from human pluripotent stem cells of FOP patients and controls

Project description:NCCs and NCC-derived MSCs were induced from FOP-iPSCs and control iPSCs, and their expresion profiles were compared. Comparison of gene expressions among hiPSCs, hESCs, hNCCs and hNC-MSCs from FOP-iPSCs and control-iPSCs.

2015-09-17 | E-GEOD-61510 | biostudies-arrayexpress

Gene expression profiles in SOX10-deficient human iPS cell-derived neural crest cells

Project description:Waardenburg syndrome (WS) is a autosomal dominant inherited disorder characterized by sensorineural hearing loss (SNHL) and pigment abnormalities. SOX10 is one of its main pathogenicity genes. In vitro generation of patient-specific induced pluripotent stem cells (iPSCs) is an effective approach for exploring the mechanisms of human disease. Here, we established an iPSCs from a WS patient with SOX10 mutation and differentiated it into induced neural crest cells (NCCs), the key cell type involves in inner ear development. Compared with control-derived iPSCs, the SOX10 mutant iPSCs showed significantly decreased efficiency of development and differentiation potential at the stage of NCCs. We then performed high-throughput RNA-seq and examined the transcriptional misregulation at each stage. The transcriptome analysis differentiated NCCs reveals widespread gene expression changes and the differentially expressed genes(DEGs) were enriched with gene ontology (GO) of neuron migration, skeletal system development, multicellular organism development, which indicate the important role of SOX10 plays in the differentiation of NCCs. It's worth noting that, a significant enrichment among the nominal DEGs for genes implicated in inner ear development was found, as well as several genes related to the inner ear morphogenesis. Based on protein-protein interaction（PPI）net work, we selected four candidate genes that may be regulated by SOX10 in inner ear development: BMP2, LGR5, GBX2 and GATA3. In conclusion, the SOX10 deficiency in this WS patient has a significant impact on the gene expression patterns during the development of NCCs. The DEGs most significantly enriched the inner ear development and morphogenesis could help explain the underlying basis for the inner ear malformation seen in WS patient.

2021-06-04 | GSE176101 | GEO

P0-cre marked neural crest cells in murine embryo

Project description:To elucidate the molecular features of craniofacial versus trunk neural crest cells (NCCs), we utilized P0-Cre/Floxed-EGFP mice that specifically label NCCs (Yamauchi et al., 1999 (PMID 10419695)). Craniofacial and trunk regions were isolated from P0-Cre/Floxed-EGFP mouse embryos at embryonic day E12.5, and dissociated cells were analyzed by flow cytometory in regard to the intensity of EGFP. In this study, we performed at least duplicate experiments for each of the four groups (Craniofacial EGFP+, Trunk EGFP+, Craniofacial EGFP-, Trunk EGFP-). Total of 9 samples.

2014-08-01 | E-GEOD-39191 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data