Project description:To investigate the potential role in mycoparasitism, microarrays were used to examine T. reesei transcript levels when confronted with a potential prey (the plant pathogen Rhizoctonia solani) before contact, during first physical contact and during overgrowth of the host. Two biological pools by condition against a common reference control each sample hybridized in dye switch. On the two biological replicates we apply on the pretreated results the linear modeling approach implemented by lmFit and the empirical Bayes statistics implemented by eBayes from the limma R package (Smyth 2004).
Project description:We perform a self hybridisation comprative genomic hybridization (CGH) in order to validate the probe tiling design we done on Trichoderma reesei. This hybridization was done using QM6a wild type strain. One biological replicate
Project description:Genome-wide RNA-seq analysis during carbon catabolite repression in Trichoderma reesei reveals a multilevel control of cellulase production and action
Project description:The morphology of filamentous fungi has a close relationship with the production of many products (e.g. industrial enzymes). In this study, we deleted gul1, which encodes a putative RNA-binding protein, in cellulolytic fungus Trichoderma reesei. The mutant showed different morphologies both on agar plates and in liquid cultures compared with its parent. RNA-seq study showed that the expression levels of genes in many biological processes were affected by gul1 deletion.
Project description:We perform a self hybridisation comprative genomic hybridization (CGH) in order to validate the probe tiling design we done on Trichoderma reesei. This hybridization was done using QM6a wild type strain.
Project description:We investigated the function of the transcription factor STE12 and found an involvement in gene expression and growth in light and darkness
