Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next generation sequencing for miRNA profile of blood sample of goiter rat model treated by quemeiteng granule

ABSTRACT: Next generation sequencing for miRNA profile of blood sample of goiter rat model treated by quemeiteng granule

PROVIDER: PRJNA871780 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21148400.fastq.gz	Fastqsanger.gz
	SRR21148401.fastq.gz	Fastqsanger.gz
	SRR21148384.fastq.gz	Fastqsanger.gz
	SRR21148385.fastq.gz	Fastqsanger.gz
	SRR21148386.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Next generation sequencing for miRNA profile of blood sample of goiter rat model treated by quemeiteng granule

Project description:MicroRNAs play important roles in the regulatory processes of occurrence and progression of goiter. In the present study, whole transcriptome sequencing analysis was applied to investigate the expression profiles of miRNAs. After comparing blood samples of propylthiouracil (PTU)-induced goiter model blood samples and quemeiteng granule (QMTG) treated groups, 1 miRNA was found to be differentially expressed. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to investigate the potential functions of the mRNAs. Our study proved QMTG relieves goiter by suppressing thyroid angiogenesis through miR-217-5p targeting of FGF2 regulating ERK pathway.

2024-05-01 | GSE211703 | GEO

Detect Microsatellite Instability Status in Blood Sample of Advanced Colorectal Cancer Patients by Next-Generation Sequencing

Project description:The method to analyze the microsatellite instability (MSI) status by next-generation sequencing (NGS) has been established to assess the deficiency of DNA mismatch repair (MMR) system. The aim of our study is to evaluate the feasibility and reliability of this NGS method by testing the circulating tumor DNA (ctDNA) in blood sample of advanced colorectal cancer patients. If the result is positive, the MSI status could be easily learned without the acquisition of tissue samples.

| 2275707 | ecrin-mdr-crc

Transcriptional profiling of dentate granule cells in 4 rat epilepsy models

Project description:Global expression profiling of epileptogenesis has been confounded by variability across laboratories, epilepsy models, tissue sampled and experimental platforms, with the result that very few genes demonstrate consistent expression changes. The present study minimizes these confounds by combining Affymetrix microarray datasets from seven laboratories, using three status epilepticus (SE) models of epilepsy in rats (pilocarpine, kainate, self-sustained SE or SSSE) and the rat kindling model. Total RNA was harvested from laser-captured dentate granule cells from 6 rats at three times during the early-to-mid latent phase that precedes epilepsy symptoms in the SE models (1, 3 and 10 days after SE), or 24 hr after the first stage 2, stage 4 and stage 5 seizure in the kindling model. Each epilepsy model was studied in two independent laboratories except SSSE. The initial goals of this study were to a) identify model-independent transcriptional changes in dentate granule cells that could point to novel intervention targets for epileptogenesis, b) characterize the basal transcriptional profile of dentate granule cells, and c) identify genes that have highly variable expression. Each experimental group consists of 6 rats (biological replicates) from one laboratory at a single time point, except for the SSSE group (6 at day 1 after SSSE, 5 controls and at day 3 after SSSE, 4 at day 10). Thus granule cells were harvested from 164 rats.

2013-06-08 | E-GEOD-47752 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of miRNA

Project description:Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare miRNA expressions of pear in two different conditions.

2017-01-26 | GSE80747 | GEO

Transcriptional profiling of dentate granule cells in 4 rat epilepsy models

2013-06-08 | GSE47752 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of miRNA

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of miRNA

| PRJNA319909 | ENA

Gene expression of cerebellar granule cell layer

Project description:We performed gene-expression analysis of mouse cerebellar granule cell layer as compared to that of Purkinje cells. DNA microarray analysis detected genes in cerebellar granule cell layer, most of which are classified into functional molecule categories. Our comparative analysis between Purkinje cells and the granule cell layer showed that the characteristic expression pattern in Purkinje cells was particularly represented by M-bM-^@M-^\the neural communication systemM-bM-^@M-^] components. Pukinje cells and granule cell layer of the mouse cerebellum were collected by laser microdissection for RNA extraction and hybridization on Affymetrix microarrays.

2013-01-17 | E-GEOD-26750 | biostudies-arrayexpress

Gene expression profiling in dentate granule cells from patients with mesial temporal lobe epilepsy with or without hippocampal sclerosis

Project description:Hippocampal sclerosis (HS) is the most common neuropathological finding of medically intractable cases of mesial temporal lobe epilepsy (MTLE), the most common form of partial epilepsy. Within the dentate gyrus, HS may be associated with granule cell dispersion and aberrant mossy fiber sprouting, and these pathological changes are accompanied by a range of molecular changes. In this study, we analyzed the gene expression profiles of dentate granule cells of MTLE patients with and without HS to show that next-generation sequencing methods can produce interpretable genomic data from RNA collected from small homogenous cell populations and to shed light on the transcriptional changes associated with HS. 12 samples of dentate granule cells from patients with mesial tempora lobe epilepsy, 5 with hippocampal sclerosis and 7 without hippocampal sclerosis. 10 samples had replicates.

2016-06-29 | E-GEOD-71058 | biostudies-arrayexpress

Next generation sequence analysis

Project description:Next generation sequence analysis

| PRJNA560769 | ENA

Next-generation sequencing reveals miRNA expression profiles of endometrial cancer cells

Project description:Abundantly expressed oncogenic miRNAs in endometrial cancer cells are identified by next-generation sequencing.

2022-07-11 | GSE134142 | GEO