Project description:Young adult N2 Caenorhabditis elegans were infected with Enterococcus faecalis or Enterococcus faecium for 8 h to determine the transcriptional host response to each enterococcal species. Analysis of differential gene expression in C. elegans young adults exposed to four different bacteria: heat-killed Escherichia coli strain OP50 (control), wild-type E. faecalis MMH594, wild-type E. faecium E007, or Bacillus subtilis PY79 (sigF::kan). Samples were analyzed at 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Brain-heart infusion agar plates (10 ug/ml kanamycin) were used.
Project description:Young adult fer-15;fem-1 Caenorhabditis elegans were infected with Staphylococcus aureus for 8 h to determine the transcriptional host response to Staphylococcus aureus. Analysis of differential gene expression in C. elegans young adults exposed to two different bacteria: E. coli strain OP50 (control), wild-type Staphylococcus aureus RN6390. Samples were analyzed at 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Keywords: response to pathogen infection, innate immunity, host-pathogen interactions
Project description:Transcriptional profiling of whole day 1 adult C. elegans, comparing animals carrying the m79 mutation in the daf-10 gene and wild-type isogenic animals. Genes that act downstream of sensory neurons to influence longevity, dauer formation and pathogen responses in Caenorhabditis elegans Manuscript abstract:
Project description:This study presents RNA sequencing data from Caenorhabditis elegans strains expressing human wild-type or G2019S-mutant LRRK2 in a pan-neuronal manner, as well as wild-type control animals. Samples were collected to investigate the transcriptomic effects of LRRK2 expression and mutation on aging-related biological processes. Total RNA was extracted from synchronized animals, and libraries were sequenced using the Illumina NovaSeq 6000 or MGISEQ-T7 platform. Clean reads were aligned to the C. elegans reference genome (WBcel235), and gene expression levels were quantified as FPKM.
Project description:CRISPR / Cas-9 mediated homologous recombination was used to fuse type IV collagen alpha chains (EMB-9 and LET-2) with the fluorescent proteins mNeonGreen and mRuby2 in Caenorhabditis elegans. L1 to L3 larvae were collected and processed for mass spectrometry. The goal of this study was to compare the abundance of each chain. The Caenorhabditis elegans strains used in this study were NK3077(qy228 [let-2::mNG] X ; qy244 [emb-9::mRuby2] III) and NK3083 (qy216 [let-2::mRuby2] X ; qy236 [emb-9::mNG] III).