Project description:2D (A549) and 3D (AIR-606) human lung epithelial cell cultures were mock treated or exposed to 100 ug/ml petroleum coke or urban air particulates (both in the PM10 range) for 16 h. Secretomes were compared for triplicate treatments for each group by isobaric tags and LC/MS/MS.
2018-03-26 | PXD005194 | Pride
Project description:Urban microbiomes of urban parks of Shanghai, China
| PRJNA395631 | ENA
Project description:Urban microbiomes of urban parks of Shanghai, China
Project description:This study aimed to shed light on the gene regulatory networks underlying plant leaf responses to air particulate matter. Our investigation focused on shrubs of Photinia x fraseri grown in pots located in two contrasting areas: a highly polluted traffic road and rural countryside within the same town (Altopascio, Lucca, Italy). RNA-seq data were related to leaf morphological traitsand air particulate matter, allowing to identify key players in modulating the capabilities of plants to phyllo-remediate high air particulate matter levels in urban environment.
Project description:In the present study, we investigate pulmonary transcriptional responses in mice following exposure in situ to ambient air in a heavily polluted urban environment. Mature C57BL/CBA male mice were caged in sheds located in an urban area near two working steel mills and a major highway in Hamilton, Ontario, Canada. Control mice were housed in the same environment but received only high-efficiency particle-filtered air. Whole lung tissues were collected from mice exposed for 3 weeks, 10 weeks or for 10 weeks followed by 6 weeks in the laboratory (16 weeks total). DNA microarrays were used to explore changes in pulmonary gene expression in mice breathing ambient air versus HEPA-filtered air. Transcriptional profiling revealed changes in the expression of genes implicated in the lipid droplet synthesis pathway (plin, dgat2, lpl, s3-12, agpat2), antioxidants (ucp1). We postulate that exposure to particulate matter adsorbed with polycyclic aromatic hydrocarbons triggers lipid droplet synthesis (holding depots for lipids and malformed/excess proteins tagged for degradation) in the lungs, which act to sequester particulates adsorbed with toxic chemicals. Increased lipid droplet synthesis could potentially lead to endogenous/stressor-induced synthesis of reactive oxygen species and activation of antioxidant mechanisms. Further investigation into the stimulation of lipid droplet synthesis in the lung in response to air pollution is warranted in order to better understand these mechanistic changes and the resulting health implications.
Project description:This study aimed to shed light on the gene regulatory networks underlying plant leaf responses to air particulate matter. Our investigation focused on autochthonous shrubs of laurel (Laurus nobilis L.) grown in pots located in two contrasting areas: a highly polluted traffic road and rural countryside within the same town (Altopascio, Lucca, Italy). RNA-seq data were related to leaf morphological traits and air particulate matter, allowing to identify key players in modulating the capabilities of plants to phyllo-remediate high air particulate matter levels in urban environment.
Project description:Human lung cells (BEAS-2B) were exposed to either liquid suspensions of 125 ug/mL urban particulate matter (NIST, SRM 1648a) or growth media only control for 24 hours at 37C, 5% CO2. We have explored the changes in expression level and subcellular abundance of key proteins involved in cellular response to air pollution stress.
2026-02-10 | PXD052537 | Pride
Project description:Fungal communities in urban park soils from Shanghai
| PRJNA556996 | ENA
Project description:Urban stormwater long read metagenomes
| PRJNA1071153 | ENA
Project description:PM2.5 metagenomes in urban China