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Live or heat-killed Lactobacillus rhamnosus aerosolization decreases adenomatous lung cancer development in a mouse carcinogen-induced tumor model

ABSTRACT: Live or heat-killed Lactobacillus rhamnosus aerosolization decreases adenomatous lung cancer development in a mouse carcinogen-induced tumor model

PROVIDER: PRJNA889756 | ENA |

REPOSITORIES: ENA

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Live or heat-killed Lactobacillus rhamnosus aerosolization decreases adenomatous lung cancer development in a mouse carcinogen-induced tumor model

Project description:One of the responsible factors involved in the progression of pre-malignant lung lesions to lung cancer is the increased immunosuppressive microenvironment of the lung, that is deeply infleunced by the microbiota. In this article, we have investigated if the perturbation of lung microbiota by antibiotics or probiotic aerosol, interferes with lung cancer development in a mouse carcinogen-induced tumor model. Specifically, the effect is mostly observed with aerosol with Lactobacillus Rhamnosus GG that results to impair lung cancer growth by promoting local immunity. This study points out to new and non-invasive strategy to treat individuals at risk of lung cancer.

2022-11-29 | GSE215317 | GEO

Live or Heat-Killed <i>Lactobacillus rhamnosus</i> Aerosolization Decreases Adenomatous Lung Cancer Development in a Mouse Carcinogen-Induced Tumor Model.

Project description:An immunosuppressive microenvironment in lung concurs to pre-malignant lesions progression to cancer. Here, we explore if perturbing lung microbiota, which contribute to immunosuppression, by antibiotics or probiotic aerosol interferes with lung cancer development in a mouse carcinogen-induced tumor model. Urethane-injected mice were vancomycin/neomycin (V/N)-aerosolized or live or dead L. rhamnosus GG (L.RGG)-aerosolized, and tumor development was evaluated. Transcriptional profiling of lungs and IHC were performed. Tumor nodules number, diameter and area were reduced by live or heat-killed L.RGG, while only a decrease in nodule diameter was observed in V/N-treated lungs. Both L.RGG and V/N reduced Tregs in the lung. In L.RGG-treated groups, the gene encoding the joining chain (J chain) of immunoglobulins was increased, and higher J chain protein and IgA levels were observed. An increased infiltration of B, NK and myeloid-derived cells was predicted by TIMER 2.0. The Kaplan-Meier plotter revealed an association between high levels of J chain mRNA and good prognosis in lung adenocarcinoma patients that correlated with increased B and CD4 T cells and reduced Tregs and M2 macrophages. This study highlights L.RGG aerosol efficacy in impairing lung cancer growth by promoting local immunity and points to this non-invasive strategy to treat individuals at risk of lung cancer.

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Comparison of Gene expression pattern between heat-killed M.leprae and live M. leprae-infected peritoneal macrophage in vivo model

2011-03-17 | GSE23749 | GEO

Comparison of Gene expression pattern between heat-killed M.leprae and live M. leprae-infected peritoneal macrophage in vivo model

Project description:Our goal is to discriminate specific genes in live M.leprae-infected peritoneal macrophages in comparison to heat-killed M.leprae infected peritoneal macrophages using microarray. Two-condition experiment, Heat-killed M.leprae infected macrophage vs. Live M.leprae infected macrophage. Biological replicates: 16 mice (control), 8 heat-killed M. leprae mice (sample 1), and 8 live M. leprae infected mice (sample 2). Independently grown and harvested from isolator. One replicate per array.

2011-03-17 | E-GEOD-23749 | biostudies-arrayexpress

Early response of bovine alveolar macrophages to infection with live and heat-killed Mycobacterium bovis

Project description:Bovine tuberculosis, caused by Mycobacterium bovis, is a disease of considerable economic importance yet comparatively little is known about the bovine immune response to the disease. Alveolar macrophages are one of the first cells to encounter mycobacteria following infection. In this experiment we investigated the early transcriptional response of bovine alveolar macrophages following infection with M. bovis. The transcriptional response to heat-killed M. bovis was also investigated to look for genes that are only differentially transcribed in response to the live organism. Five-condition experiment, uninfected, live and heat-killed M. bovis-infected bovine alveolar macrophages from five cattle infected for two and four hours. Comparisons were within animal. Dye swaps were incorporated into the design.

2010-01-30 | E-GEOD-19237 | biostudies-arrayexpress

Early response of bovine alveolar macrophages to infection with live and heat-killed Mycobacterium bovis

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Project description:DNA methylation of 23 familial adenomatous polyposis tumors. Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across 485,577 CpG sites.

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Heat-killed Fusarium-elicited W22-stem time_course

Project description:We aim to examine dynamic defense transcript changes in maize stems following elicitation with heat-killed Fusarium hyphae.

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DNA methylation analysis in familial adenomatous polyposis

Project description:DNA methylation analysis in familial adenomatous polyposis

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