Project description:Visium Spatial Gene Expression by the 10X genomics protocol was performed on 2 sections of human DRG tissue to elucidate the spatial transcriptomic organization.
Project description:Bulk RNA sequencing was performed to compare mRNA expression profiles across six experimental conditions: monoculture of primary dorsal root ganglion (DRG) neurons isolated from C57BL/6J mice; conditioned medium from EO771 cells applied to DRG neurons; and direct co-culture of EO771 and DRG neurons followed by fluorescence-activated cell sorting (FACS) to DRG neuron population. The aim was to identify transcriptional programs regulated by tumor–neuron interactions, including acute gene expression changes, intercellular signaling pathways, and broader pathway-level alterations. The dataset is intended for differential expression analysis, pathway enrichment, and hypothesis generation for downstream functional studies.
Project description:This dataset comprises 10X Visium spatial transcriptomics data from the pig dorsal root ganglia (DRG) of female pigs. It aims to map the spatial gene expression patterns within the DRG, offering insights into the molecular identity of neuronal populations, including nociceptors. This work provides a unique spatial perspective on gene expression, facilitating a deeper understanding of DRG molecular architecture and its implications in sensory processing.
Project description:Changes in microRNA (miRNA) expression in the mouse L4 and L5 dorsal root ganglion following unilateral sciatic nerve transection. The timepoint of 7 days post-axotomy was chosen to capture miRNA expression profiles at a time when the injured neurons were beginning to regenerate. Two condition experiment, paired control DRG vs axotomised DRG following unilateral sciatic nerve transection. 3 biological replicates, one replicate per array. Dye swap in Replicate 2.
Project description:We have employed a single cell sequencing approach using 10x Genomics scRNAseq to study murine dorsal root ganglion (DRG) sensory neurons contribution to cancer-induced bone pain. DRG are the hub of sensory neurons and pain signaling. These findings establish a non-tumor-bearing reference dataset.
Project description:We generated whole-genome gene expression profiles of dorsal root ganglion (DRG) neurons following nerve damage. DRG neurons extend one peripheral axon into the spinal nerve and one central axon into the dorsal root. The peripheral axon regenerates vigorously, while in contrast the central axon has little regenerative capacity. For this study, two groups of animals were subjected either to sciatic nerve (SN) or dorsal root (DR) crush, and at 12, 24, 72 hours and 7 days after the crush, lumbar DRGs L4, L5 and L6 were dissected and total RNA was extracted. For each time point after lesion, three biological replicate RNA samples were hybridized together with the common reference sample consisting of labeld RNA pooled from three unlesioned animals.
Project description:We generated whole-genome gene expression profiles of dorsal root ganglion (DRG) neurons following nerve damage. DRG neurons extend one peripheral axon into the spinal nerve and one central axon into the dorsal root. The peripheral axon regenerates vigorously, while in contrast the central axon has little regenerative capacity. For this study, two groups of animals were subjected either to sciatic nerve (SN) or dorsal root (DR) crush, and at 12, 24, 72 hours and 7 days after the crush, lumbar DRGs L4, L5 and L6 were dissected and total RNA was extracted.
Project description:Visium spatial RNA sequencing was performed on 16 human dorsal root ganglia (DRG) tissue sections collected from 7 different DRGs, from 6 human subjects with a history of diabetes.