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ABSTRACT: Corynebacterium glutamicum DactA_IxylAB and ALE-derived mutant

PROVIDER: PRJNA895044 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22069536_1.fastq.gz	Fastqsanger.gz
	SRR22069537_1.fastq.gz	Fastqsanger.gz
	SRR22069537_2.fastq.gz	Fastqsanger.gz
	SRR22069538_1.fastq.gz	Fastqsanger.gz
	SRR22069539_1.fastq.gz	Fastqsanger.gz

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Comparison of Corynebacterium glutamicum Δcg2699 with WT

Project description:To identify genes which are differentially expressed in Corynebacterium glutamicum in the cg2699 deletion strain, we performed DNA microarray analyses of C. glutamicum Δcg2699 compared to the WT.

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Project description:Corynebacterium glutamicum GlxR is a homolog of the cAMP receptor protein. Although over 200 GlxR binding sites in the C. glutamicum genome are predicted in silico, studies on the GlxR physiological function have been hindered by the severe growth defects of a glxR mutant. This study comprehensively identified the GlxR regulon by chromatin immunoprecipitation in conjunction with microarray (ChIP-chip) analyses. In total, 209 regions were detected as in vivo GlxR binding sites. Moreover, ChIP-chip analyses showed that GlxR was still able to interact with its target sites in a deletion mutant of cyaB, the sole adenylate cyclase gene in the genome, even though binding affinity was markedly decreased.

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Project description:In this study, disruption and overexpression of sigD were performed in Corynebacterium glutamicum and analyzed by transcriptome sequencing (RNA-seq) to understand the SigD regulon in C. glutamicum. For the effect of sigD overexpression, the relative abundance of mRNA was compared in WT(pVWEx1-sigD) without IPTG or with 50 M of IPTG. For the effect of sigD disruption, the abundance was compared between the sigD disrupted mutant and the wild type strain.

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RNAseq analysis of global gene expression patterns in Corynebacterium glutamicum Adaptive Laboratory Evolution (ALE) strains GluA T5 and GluA T7 with accelerated growth and glutarate production.

Project description:The Adaptive Laboratory Evolution (ALE) experiment allowed to select Corynebacterium glutamicum strain GluA T5, which grew faster and produced more glutarate than the parent strain GluA T0, and strain GluA T7 that grew as fast as GluA T5, but produced more 5AVA than GluA T5. To explore differences in the gene expression in the evolved strains, C. glutamicum GluA T0, GluA T5 and GluA T7 biological triplicates were grown in CGXII minimal medium with 4% (w/v) glucose supplemented with 1 mM IPTG. Exponentially growing cells were harvested by centrifugation (14000 × g, 1 min) and kept at -80°C. RNA isolation, purification and quality control was performed as described [82] and the high quality RNA (RNA integrity number > 9.0) was kept at -80°C until further use. Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the ribosomal RNA molecules from the isolated total RNA. Preparation of cDNA libraries were performed according to the manufacturer’s instructions of TruSeq stranded mRNA Kit (Illumina, San Diego, USA). Subsequently, each cDNA library was sequenced on a HiSeq1500 (2 x 70nt PE rapid v2) and NextSeq 500 (2 x 75nt PE mid output v2.5) Sequencer system (Illumina, San Diego, USA).

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