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Expression data from human primary monocyte derived immature dendritic cells (iDC)

ABSTRACT: Expression data from human primary monocyte derived immature dendritic cells (iDC)

PROVIDER: PRJNA903375 | ENA |

REPOSITORIES: ENA

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Expression data from human primary monocyte derived immature dendritic cells (iDC)

Project description:During HIV-1 infection, iDCs contribute to mounting an immune response and to viral dissemination, however most of the studies addressing iDCs-HIV-1interactions have been performed in standard 2D suspension cultures which do not reflect the 3D architecture of tissues in vivo. To overcome this limitation and to understand the role that the 3D environemt has we studied iDC biology as well as iDC-HIV-1 functional interactions in 3D collagen matrix comparing 3D collagen cultures to standard 2D suspension cultures. We used microarray data to analyze how the 3D environment affects the expression profile of monocyte derived iDCs under basal condition, LPS stimulation and in response to HIV-1 infection

2023-03-28 | GSE218367 | GEO

Transcriptomic analysis of donor matched primary human immature dendritic cells and monocyte-derived Langerhans cells

Project description:Transcriptomic analysis of donor matched primary human immature dendritic cells and monocyte-derived Langerhans cells

| PRJNA809703 | ENA

Transcriptome modulation analysis of cytomegalovirus-infected immature monocyte-derived dendritic cells

Project description:Analysis of dendritic cells (DCs) at 6h and 48h after infection with cytomegalovirus (CMV) versus non infected. CMV tends to induce immunosuppression followed by lasting immunity. DCs appear to play a role in this effect. Results provide insights into CMV/DC interactions and suggest mechanisms for CMV-regulated early immune response. Keywords: gene expression array-based, count In the present study, immature monocyte-derived dendritic cells were generated from CMV neg healthy blood donors and infected or not with an endotheliotropic CMV strain. Gene expression modulation by the CMV infection was studied in a time course assay (non-infected - NI - vs 6h or 48h post-infection. Patient C has no 48h sample

2009-01-22 | E-GEOD-14490 | biostudies-arrayexpress

Expression data from immature dendritic cells (iDC) expressing HIV-1 Tat alleles and mutants

2013-06-01 | GSE42924 | GEO

microRNA expression signature of monocyte-derived dendritic cells (DCs) from peripheral blood mononuclear cells (PBMCs)

Project description:In order to detect the microRNA expression profile of in vitro generated dendritic cells , purified monocytes from PBMCs were used as dendritic cell (DCs) precursors and were cultured in medium with cocktail for differentiation and maturation to immature dendritic cells (iDCs) and mature dendritic cells (mDCs). microRNA samples were isolated from precursor, iDCs and mDCs and used for microarray-based microRNAs expression profiles. To generate enough amount of immature DC (iDCs) and mature DCs (mDCs), monocytes were differentiated with GM-CSF and rhIL-4 for 2 days and maturated in the presence of TNF-α, IL-1β, IL-6 and PGE2 for another 2 days. With the anticipation to insight developmental-stage-specific microRNAs with potential functions related to monocyte derived DCs, global microRNAs expression profiling was set using microarray technology.microRNA expression profiles were performed in triplicate independent experiments starting for 3 groups of precursor, iDC and mDC generated from different blood donors.

2013-01-28 | E-GEOD-15036 | biostudies-arrayexpress

Gene expression profiling of monocyte-derived dendritic cells (DCs) from peripheral blood mononuclear cells (PBMCs)

Project description:In order to detect the gene expression profile of in vitro generated dendritic cells , purified monocytes from PBMCs were used as dendritic cell (DCs) precursors and were cultured in medium with cocktail for differentiation and maturation to immature dendritic cells (iDCs) and mature dendritic cells (mDCs). Total RNA samples were isolated from precursor, iDCs and mDCs and used for microarray-based gene expression profiles. To generate enough amount of immature DC (iDCs) and mature DCs (mDCs), monocytes were differentiated with GM-CSF and rhIL-4 for 2 days and maturated in the presence of TNF-α, IL-1β, IL-6 and PGE2 for another 2 days. With the anticipation to insight developmental-stage-specific genes with potential functions related to monocyte derived DCs, global gene expression profiling was set using microarray technology.gene expression profiles were performed in triplicate independent experiments starting for 3 groups of precursor, iDC and mDC generated from different blood donors.

2013-01-28 | E-GEOD-15075 | biostudies-arrayexpress

Transcriptomic analysis of donor matched primary human immature dendritic cells and monocyte-derived Langerhans cells

Project description:Donor matched immature dendritic cells and Langerhans cells were generated from CD14+ monocytes to show the validity of our Langehans cell model.

2022-11-20 | GSE197299 | GEO

Expression data from immature dendritic cells (iDC) expressing HIV-1 Tat alleles and mutants

Project description:HIV-1 Tat induces the expression of interferon (IFN)-inducible genes in immature dendritic cells (iDC) in the absence of IFN production. We evaluated how three alleles of Tat and some Tat mutants differ in cellular gene modulation and whether a similar gene induction pattern could be detected by treating cells with IFNM-bM-^@M-^Ys. The three alleles and mutants, with the exception of mutants TatSF21-47 and TatSF2G48-R57A that do not localize in the nucleous, modulated to different degrees IFN-inducible genes without concomitant induction of IFNM-bM-^@M-^Ys. The first exon TatSF21-72 and the minimal transactivator TatSF21-58, all induced genes to a significantly greater extent than full-length Tat. The 2nd exon appears to diminish the gene modulation that can be observed when the first exon alone is expressed. We investigated the effect of various Tat alleles and mutants on host cell gene expression in immature dendritic cells (iDC). The cells were infected with adenoviruses expressing the Tat constructs. The Tat alleles and mutants are under a tetracycline inducible promoter and are expressed only in cells co-infected with Ad-tTA, which expresses the tetracycline responsive transactivator. 10e6 iDC were infected with 5 plague forming units per cell of the adenoviruses and cells were collected 10 and 20 hours post-infection. RNA was isolated and mRNA expression levels were analyzed by microarrays. As controls we used cells that were left uninfected and cells that were infected with Ad-LacZ, which expresses beta-galactosidase.

2013-06-01 | E-GEOD-42924 | biostudies-arrayexpress

Genome-wide analysis of mRNA transcriptomes in dendritic cell and exosome-treated dendritic cell

Project description:The present research aimed to demonstrate that pancreatic cancer related miRNAs can be transferred from exosome to dendritic cells and influence mRNA expression, inducing immune tolerance of dendritic cells .Immature dendritic cells were induced from peripheral blood monocytes and stimulated by exosomes collected from PANC-1 cell culture.Genome-wide mRNA chip were performed in iDC and exosome stimulated iDC to detect the change of mRNA expression level.

2015-03-20 | GSE67020 | GEO

miRNA expression profiling of human monocyte-derived dendritic cells (moDCs) during maturation

Project description:miRNA expression profiling of human monocyte-derived dendritic cells (moDCs) during maturation. Immature, 4h and 16h LPS-activated moDCs were used.

2009-07-27 | GSE13369 | GEO

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