Ontology highlight
ABSTRACT:
PROVIDER: PRJNA912168 | ENA |
REPOSITORIES: ENA
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Project description:The effects of changes in bioprocessing parameters (temp, pH, DO) on anti-IL8 IgG1 producing Chinese hamster ovary (CHO) cells were studied on the level of host cell proteome expression using TMT-SPS-MS3.
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Project description:Two clones of Chinese hamster ovary (CHO) cells were cultured in 2 different chemical difined custom media. Transcriptional profile were compeared between clones and media.
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Project description:A synthesized Chinese Hamster Ovary (CHO) cell genome-scale model (GeM) consisting of iCHO2291 (Yeo. et al 2020) coupled with the secretory machinery presented in iCHO2048 (Gutierrez et al. 2020). This model is the most comprehensive CHO GeM to date, boasting the highest number of genes compared to any previous CHO GeM.
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Project description:Chinese Hamster Ovary cell line transcriptome sequencing
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Project description:Sodium butyrate (NaBu) is well-known for its capacity to hinder cellular growth and act as a histone deacetylase inhibitor. It is commonly employed in the cultivation of recombinant Chinese hamster ovary (CHO) cell cultures to boost the production of specific proteins, such as antibodies. In this investigation, two types of CHO cell lines, namely K1 and DG44, along with their respective mAb-producing lines, K1-Pr and DG44_Pr, were cultured with or without NaBu. To analyze the proteome, a SWATH-based profiling method was utilized. The outcomes were assessed using Spectronaut 17, while STRING and Gene Ontology pathway analyses were performed using Cytoscape. The analysis confirms the known effects of NaBu on mAbs production, adding information on redox homeostasis of the cells.
2025-05-06 | PXD048575 | Pride
Project description:Gene expression is a key determinant of phenotypes that made Chinese Hamster Ovary (CHO) cells, with their human-like glycosylation profile and high protein titers, one of the most widely used cells for the production of therapeutic proteins and biopharmaceuticals. Engineering CHO gene expression thus holds a key to improve drug quality and cost effective production. However, the success of engineering gene expression or ectopic activation of silent genes to optimize desired pathways requires accurate annotation of the underlying regulatory elements and the transcription start site (TSS). Unfortunately, to date, most TSSs of CHO-expressed genes and the ~50% of hamster genes that are silent in CHO were computationally predicted and are frequently inaccurate. To oust this hurdle, we report revised TSSs annotations for 15,308 Chinese Hamster genes and 4,145 non-coding RNAs based on experimental data from CHO K1 cells and 10 hamster tissues. In the example of the glycosyltransferase gene Mgat3, we further demonstrate how accurate annotations readily facilitate activating silent genes by CRISPRa. Together, we envision that our annotation and data from the Chinese Hamster will provide a rich resource for the CHO community, improve genome engineering efforts and additionally aid comparative and evolutionary studies.
2020-10-06 | GSE159044 | GEO
Project description:Mammalian cells are critical hosts for the production of most therapeutic proteins and many proteins for biomedical research. While cell line engineering and bioprocess optimization have yielded high protein titers of some recombinant proteins, many proteins remain difficult to express. Here we use systems biology methods to deciper the factors influencing yields in Chinese hamster ovary (CHO) cells as they produce proteins from the human secretome.
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Project description:Investigation of Trancriptome and methylome changes in Chinese Hamster Ovary cells after single cell cloning
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