Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression of C28/I2 cells in monolayer and spheroid culture

ABSTRACT: Gene expression of C28/I2 cells in monolayer and spheroid culture

PROVIDER: PRJNA931934 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23347789.fastq.gz	Fastqsanger.gz
	SRR23347790.fastq.gz	Fastqsanger.gz
	SRR23347791.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

Gene expression of C28/I2 cells in monolayer and spheroid culture

Project description:Here we described the transcriptional differences of chondrocytes in monolayer and spheroid cultures.

2023-04-18 | GSE224598 | GEO

Effects of piR_019914 on gene expression in C28/I2 cells

Project description:In order to study the role of piR_019914 in C28/I2 cells, we established piR_019914_mimics to overexpress piR_019914 in C28/I2 cells We then performed gene expression profiling analysis using data obtained from RNA-seq of piR_019914 overexpression C28/I2 cells and mimics_NC C28/I2 cells.

2023-03-27 | GSE227945 | GEO

Effect of Overexpression of DPP4 on Gene Expression in C28/I2 Chondrocyte Cells

Project description:We report here the overexpression of DPP4 in C28/I2 chondrocyte cells to investigate the changes induced by elevated levels of this gene. DPP4 is known as a marker of cellular senescence, yet its regulatory mechanisms remain unclear. In this study, we utilized the C28/I2 cell line to overexpress DPP4 and analyzed the resultant effects on gene expression and cellular behavior. Our findings reveal that DPP4 overexpression influences several pathways associated with chondrocyte function and senescence. Specifically, we observed alterations in pathways related to extracellular matrix organization, inflammatory response, and cellular aging. These results suggest that DPP4 plays a significant role in the modulation of chondrocyte senescence and may provide new insights into the mechanisms of cellular aging. Taken together, our data provide a molecular framework for understanding how DPP4 overexpression impacts chondrocyte physiology and offers potential targets for therapeutic intervention in age-related cartilage diseases.

2025-02-19 | GSE273261 | GEO

Expression data from normal human spheroid-forming keratinocytes in monolayer mass culture and from corresponding cornified-like spheroid ring structures.

Project description:3-D suspension culture differs considerably from monolayer culture in regards to cell architecture, cell to cell contact, and cell to matrix interactions. These factors play a regulatory role in epithelial stem cell homeostasis and influence epidermal stem cells on their decision to remain quiescent or to activate self-renewal. We used microarrays to determine the global programme of gene expression underlying spheroid ring formation and identified distinct gene programs involved in this process.

2021-01-31 | GSE94244 | GEO

Proteomics of ovarian cancer stem like cells A2780: Monolayer vs Spheroid

Project description:Proteomics of ovarian cancer cell line A2780 in 2D monolayer and in 3D spheroid culture.

2025-05-10 | MSV000097853 | MassIVE

Proteomics of ovarian cancer stem like cells SKOV-3: Monolayer vs Spheroid

Project description:Proteomics of ovarian cancer cell line SKOV-3 in 2D monolayer and in 3D spheroid culture

2025-07-09 | MSV000098472 | MassIVE

Gene expression of C28/I2 cells with FABP4

Project description:Gene expression of C28/I2 cells with FABP4

| PRJNA945356 | ENA

Gene expression analysis in C28/I2 cells: Comparison of OENC, DPP4 Overexpression, and DPP4 Overexpression with 4,5-Dicaffeoylquinic Acid Treatment Using RNA-Seq

Project description:To examine the effects of DPP4 overexpression and its modulation by 4,5-Dicaffeoylquinic acid on chondrocytes, we established C28/I2 cell lines with DPP4 overexpression (DPP4OE), control empty vector (OENC), and DPP4OE treated with 4,5-Dicaffeoylquinic acid. Gene expression profiling analysis was performed using RNA sequencing (RNA-seq) with biological replicates to investigate the global transcriptomic changes associated with these conditions.

2025-02-19 | GSE273447 | GEO

Effects of piR_019914 on gene expression in C28/I2 cells

Project description:Effects of piR_019914 on gene expression in C28/I2 cells

| PRJNA947546 | ENA

Proteome profiling of myogenic differentiation of alveolar mucosa multipotent mesenchymal stromal cells revealed an unexpected deviation between 2D monolayer and 3D spheroid model cultures.

Project description:Recently, it has been proposed to employ three-dimensional cultivation of cell in spheroids that contributes more proper physiological conditions in matrix extracellular space architecture and cell morphology accompanied by appropriate intracellular biomechanical organization of cytoskeleton and energy generation apparatus adaptation. In this research we presented the data of proteome survey for cells growing in monolayer and in spheroids that were induced in myogenic way of differentiation and reached terminal stage in seven days. Semi-quantitative profiling of the dynamic changes in proteins abundance depicted strict advantage of spheroid manner cultivation in contrast to monolayer. Cells cultured in spheroid were significantly enriched in proteins of mitochondria biogenesis, respiratory chain proteins, extracellular proteins and cytoskeleton. Most of signal transducers involved in active proliferation and early stages of differentiation were inhibited in spheroid cultures unlike monolayer. The obtained data were broadly consistent with known molecular mechanisms of myogenesis in transcripts level and reflected strong advantage of spheroid cultivation conditions.

2021-11-15 | PXD015410 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data