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Bacteriophage

ABSTRACT: Bacteriophages lambda, T7, T5, T2 Genome sequencing

PROVIDER: PRJNA933262 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23445400.fastq.gz	Fastqsanger.gz
	SRR23445401.fastq.gz	Fastqsanger.gz
	SRR23445402.fastq.gz	Fastqsanger.gz
	SRR23445403.fastq.gz	Fastqsanger.gz
	SRR23445404.fastq.gz	Fastqsanger.gz

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1 Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity

Project description:On-demand biomanufacturing has the potential to improve healthcare and self- sufficiency during space missions. Cell-free transcription and translation reactions combined with DNA blueprints can produce promising therapeutics like bacteriophages and virus-like particles. However, how space conditions affect the synthesis and self-assembly of such complex multi- protein structures is unknown. Here, we characterize the cell-free production of infectious bacteriophage T7 virions under simulated microgravity. Rotation in a 2D-clinostat increased the number of infectious particles compared to static controls. Quantitative analyses by mass spectrometry, immuno-dot-blot and real-time PCR showed no significant differences in protein and DNA contents, suggesting enhanced self-assembly of T7 phages in simulated microgravity. While the effects of genuine space conditions on the cell-free synthesis and assembly of bacteriophages remain to be investigated, our findings support the vision of a cell-free synthesis-enabled “astropharmacy”.

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Combined Solution and Crystal Methods Reveal the Electrostatic Tethers That Provide a Flexible Platform for Replication Activities in the Bacteriophage T7 Replisome

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