Project description:To identify the mechanisms regulating osteoblasts and osteoclasts in Dmp1Cre.Socs3f/f.Csf3r-/- mice, we performed a proteomic analysis of their bone marrow compared to littermate Dmp1Cre.Socs3f/f controls.
Project description:Effects of SOCS3 on the transcriptional response of bone marrow-derived macrophages to IL-6. Fetal liver cells from SOCS3+/+ or SOCS3-/- embryos were used to reconstitute recipient mice. Donor derived bone marrow from these mice was differentiated to macrophages. Macrophages were either unstimulated, or stimulated for 100 or 400 minutes with 10 ng/ml IL-6. Keywords = macrophage, SOCS3, IL-6, interferon Keywords: repeat sample
Project description:This study examines the impact of tumor presence on bone marrow hematopoiesis in mouse models of liver cancer. Using a Hepa1-6 xenograft model in C57BL/6 mice, hematopoietic stem and progenitor cell populations were analyzed by flow cytometry. Although overall HSPC frequencies did not differ between tumor-bearing and control mice, tumor size positively correlated with HSPC numbers. RNA-seq comparison between xenograft and DEN-induced liver tumors revealed distinct transcriptomic profiles, indicating that xenograft tumors do not fully recapitulate the liver tumor microenvironment.
Project description:We speculate that the genes that acquired mutations in liver cancer tissues might be preferentially and actively transcribed in hepatic lineage cells. Therefore, to examine whether the mutated genes in liver cancer tissues in mice were transcribed at relatively higher levels in liver-lineage cells compared with hematopoietic lineage cells, we aim to analyze and compare the gene expression profiles in the adult liver, fetal liver and bone marrow.
Project description:RATIONALE: Radiation therapy uses high-energy x-rays to damage cancer cells. Drugs used in chemotherapy use different ways to stop cancer cells from dividing so they stop growing or die. Combining chemotherapy with bone marrow transplantation may allow the doctor to give higher doses of chemotherapy drugs and kill more tumor cells.
PURPOSE: Phase II trial to study the effectiveness of bone marrow transplantation in treating patients who have hematologic cancer.
Project description:This phase II trial studies how well giving fludarabine phosphate, cyclophosphamide, tacrolimus, mycophenolate mofetil and total-body irradiation together with a donor bone marrow transplant works in treating patients with high-risk hematologic cancer. Giving low doses of chemotherapy, such as fludarabine phosphate and cyclophosphamide, and total-body irradiation before a donor bone marrow transplant helps stop the growth of cancer cells by stopping them from dividing or killing them. Giving cyclophosphamide after transplant may also stop the patient’s immune system from rejecting the donor’s bone marrow stem cells. The donated stem cells may replace the patient’s immune system cells and help destroy any remaining cancer cells (graft-versus-tumor effect). Sometimes the transplanted cells from a donor can also make an immune response against the body’s normal cells. Giving tacrolimus and mycophenolate mofetil after the transplant may stop this from happening
Project description:In order to comprehensively characterize bone marrow mesenchymal cells after myeloablation, single-nuclei RNA sequencing was performed on bone marrow adipocytes and bone marrow stromal cells isolated from sublethally-irradiated mice.