Project description:To examine how the Arabidopsis root development responds to the Rhizobium sp. IRBG74 treatment at the molecular level, we performed RNA-seq experiments. Our RNA-seq results suggest that expression of genes mainly involved in auxin signaling, cell wall and cell membrane integrity and transport is altered in response to colonization by Rhizobium sp. IRBG74.
Project description:To investigate the gene expression levels of Medicago truncatula roots after beneficial fungi Gongronella sp. w5 inoculated.Gongronella sp. w5 promoted M. truncatula growth and caused the accumulation of sucrose in M. truncatula root tissue at 16 day-post-inoculation (dpi) without invading into the root cells. The transport of photosynthetic product sucrose to the rhizosphere by M. truncatula root cells was accelerated by upregulating the SWEET gene.
Project description:Root foraging strategy of wheat for potassium (K) heterogeneity is based on special gene expressions. Low-K responsive genes, such as peroxidases, mitochondrion, transcription factor activity, calcium ion binding and respiration, up-regulated in Sp. NK rather than in Sp. LK. Methyltransferase activity, protein amino acid phosphorylation, potassium ion transport, protein kinase activity genes were found among down-regulated genes in Sp. LK. We used microarrays to detail the global programme of gene expression underlying wheat root foraging strategy and identified distinct classes of up-regulated and down-regulated genes during this process.
Project description:The molecular regulation of root initiation in monocots is still poorly understood. We report the development of the auxin-based crown-root inducible system (CRIS) in barley (Hordeum vulgare L.). Using this system coupled to RNAseq, we investigated the molecular events occurring during the early steps of crown-root (CR) initiation. The sequencing on the Illumina NovaSeq6000 platform yielded over 123 Gb data, representing more than 408 million reads. Using the last released barley annotation (Morex v2 Gene Models, 2019), we found that 427 genes were downregulated by auxin whereas 764 genes were upregulated. This study provides the first analysis of early event of crown-root initiation in cereals. It will serve as comparison with known genes in rice, maize and other cereals, and could be used in metagenomics studies.
Project description:Upon exposure to unfavorable environmental conditions, plants need to respond quickly to maintain their homeostasis. For instance, physiological, biochemical and transcriptional changes occur during plant-pathogen interaction. In the case of Vanilla planifolia Jacks., a worldwide economically important crop, it is susceptible to Fusarium oxysporum f. sp. vanillae. This pathogen causes root and stem rot in vanilla plants that lead to plant death. To investigate how vanilla plants, respond at the transcriptional level upon infection with F. oxysporum f. sp. vanillae, here we employed the RNA-Seq approach to analyze the dynamics of whole-transcriptome changes during two-time frames of the infection. Analysis of global gene expression profiles indicated that the major transcriptional change occurred at 2 dpi, in comparison to 10 dpi. Whereas 3420 genes were found with a differential expression at 2 dpi, only 839 were identified at 10 dpi. The analysis of the transcriptional profile at 2 dpi suggests that, among other responses, vanilla plants prepare to counter the infection by gathering a pool of translational regulation-related transcripts. The screening of transcriptional changes of V. planifolia Jacks upon infection by F. oxysporum f. sp. vanillae provides insights into the plant molecular response, particularly the upregulation of ribosomal proteins at early stages. Thus, we propose that the plant-pathogen interaction between V. planifolia Jacks and F. oxysporum f. sp. vanillae causes a transcriptional reprogramming coupled with a translational regulation. Altogether, this study provides the identification of molecular players that could help to fight the most damaging disease of vanilla.
