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Sciatic nerve crush regulation of ribosome-associated RNA in DRG neurons

ABSTRACT: Sciatic nerve crush regulation of ribosome-associated RNA in DRG neurons

PROVIDER: PRJNA962455 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24322456_1.fastq.gz	Fastqsanger.gz
	SRR24322456_2.fastq.gz	Fastqsanger.gz
	SRR24322457_1.fastq.gz	Fastqsanger.gz
	SRR24322457_2.fastq.gz	Fastqsanger.gz
	SRR24322458_1.fastq.gz	Fastqsanger.gz

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Sciatic nerve crush regulation of ribosome-associated RNA in DRG neurons

Project description:Nerve injury alters transcription and translational processes in cell-type specific manner.Here we probe the effect of sciatic nerve injury on ribosome associated RNA specifically in sensory neurons.Mice were subjected to crush of the sciatic nerve in one side, while the other nerve was spared and used as naive control condition.

2025-05-16 | GSE230722 | GEO

Sciatic nerve crush regulation of ribosome-associated RNA in DRG neurons, alternative polyadenylation analytsis [DRGinjuryRiboTag2018-QuntSeq72h]

Project description:Nerve injury alters transcription and translational processes in cell-type specific manner.Here we probe the effect of sciatic nerve injury on alternative polyadenylation of ribosome associated RNA specifically in sensory neurons.Mice were subjected to crush of the sciatic nerve in one side, while the other nerve was spared and used as naive control condition.

2025-05-16 | GSE233823 | GEO

Sciatic nerve crush upregulation of B2-SINE expression at specific genomic loci

Project description:To map the loci of B2-SINE expressed in DRG which are regulated by sciatic nerve injury, paired-end RNAseq was carried on DRG neurons following sciatic nerve injury at 24h and 72h (3d) after injury. Mice were subjected to crush of the sciatic nerve in one side, while the other nerve was spared and used as naive control condition.

2025-05-16 | GSE230720 | GEO

Sciatic nerve crush regulation of RNA alternative poly-adenylation

Project description:Poly-adenylation regulates post-transcriptional processing and localization of mRNA. To study the effect of nerve injury on poly-adenylation across the transcriptome, 3'-end RNAseq was carried on DRG neurons following sciatic nerve injury. Mice were subjected to crush of the sciatic nerve in one side, while the other nerve was spared and used as naive control condition.

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Sciatic nerve crush regulation of RNA alternative poly-adenylation [DRGinjury_2019-QuantSeq_Rev_24h]

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Sciatic nerve crush regulation of ribosome-associated RNA in DRG neurons, alternative polyadenylation analytsis [DRGinjuryRiboTag2018-QuntSeq72h]

Project description:Sciatic nerve crush regulation of ribosome-associated RNA in DRG neurons, alternative polyadenylation analytsis [DRGinjuryRiboTag2018-QuntSeq72h]

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In vivo analysis of camptothecin effect in primary DRG neuron after sciatic nerve injury

Project description:Analysis of gene expression in injured primary DRG with or without camptothecin (CPT) treatment after sciatic nerve crushing may help us identify critical molecular pathways related to axon regeneration. We performed RNA-sequencing of (i) Naive primary DRG tissues without injury, (ii) Primary DRG tissues with vehicle treatment different time-points (18, 24, 36 hours) after sciatic nerve injury, and (iii) Primary DRG tissues with camptothecin treatment different time-points (18, 24, 36 hours) after sciatic nerve injury.

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Project description:Sciatic nerve ligation was performed on cohorts of 2-month and 24-month old animals. Resulting gene-expression data were generated from sciatic nerve 1 and 4 days after injury compared to naïve animals. Results show differences in sciatic nerve responses with normal aging. Total RNA taken from sciatic nerves from 2-month and 24-month old animals at either day 0, 1 and 4 after sciatic nerve crush injury.

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DLK-gated responses in motoneurons following sciatic nerve crush

Project description:To profile downstream gene-expression changes regulated by DLK, we profiled motoneurons in the lumbar spinal cord using RiboTag, following sciatic nerve crush in both control and Dlk conditional knockout (Dlk ΔMN) mice. We found that DLK regulates the expression of many secreted proteins, which have the potential to influence the behavior of other cells, including the immune system.

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Expression changes in L4 DRG initiated by sciatic nerve injury

Project description:Expression of L4 DRG after sciatic nerve cut compared to contralateral uninjured DRGs. The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech. The ID of this project in Genentech's ExpressionPlot database is PRJ0003322 Keywords: Expression profiling by array

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