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Redox-sensitive High-mobility group box-1 isoforms contribute to liver fibrosis progression and resolution in mice

ABSTRACT: Redox-sensitive High-mobility group box-1 isoforms contribute to liver fibrosis progression and resolution in mice

PROVIDER: PRJNA977696 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24765600_1.fastq.gz	Fastqsanger.gz
	SRR24765600_2.fastq.gz	Fastqsanger.gz
	SRR24765601_1.fastq.gz	Fastqsanger.gz
	SRR24765601_2.fastq.gz	Fastqsanger.gz
	SRR24765602_1.fastq.gz	Fastqsanger.gz

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Redox-sensitive high-mobility group box-1 isoforms contribute to liver fibrosis progression and resolution in mice

Project description:Redox-sensitive high-mobility group box-1 isoforms contribute to liver fibrosis progression and resolution in mice

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Redox-sensitive high-mobility group box-1 isoforms contribute to liver fibrosis progression and resolution in mice

Project description:Since oxidative stress plays a major role in liver fibrosis and induces post-translational modifications of proteins, we hypothesized that redox-sensitive HMGB1 isoforms contribute to liver fibrosis progression and resolution. This study is significant because a rise in [H] HMGB1 could flag 'patient at risk', the presence of [O] HMGB1 could suggest 'disease in progress or active scarring', while the appearance of [SO3] HMGB1 could point at 'resolution under way'. The latter could be used as a readout for response to pharmacological intervention with anti-fibrotic agents.

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Redox-sensitive High-mobility group box-1 isoforms contribute to liver fibrosis progression and resolution in mice

Project description:To study role of different HMGB1 isoforms in liver fibrosis and resolution

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Investigating the effects of different HMGB1 redox isoforms on macrophage polarization

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RNA sequencing of high mobility group box 1-treated melanocytes [hmgb1]

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Redox-sensitive bZIP68 plays a role in balancing stress tolerance with growth in Arabidopsis

Project description:Perturbation of the cellular redox state by stress conditions is sensed by redox-sensitive proteins so that the cell can physiologically respond to stressors; however, the mechanisms linking sensing to response remain poorly understood in plants. Here we report that the transcription factor bZIP68 underwent in vivo oxidation in Arabidopsis cells under oxidative stress which is dependent on its redox-sensitive Cys320 residue. bZIP68 is primarily localized to the nucleus under normal conditions in Arabidopsis seedlings and oxidative stress reduces its accumulation in the nucleus and increases its cytosolic localization. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) revealed that bZIP68 primarily binds to promoter regions containing the core G-box (CACGTG) or G-box-like motif of the genes involved in abiotic and biotic stress responses, photosynthesis, biosynthetic processes, and transcriptional regulation. The bzip68 mutant displayed slower growth but enhanced tolerance to oxidative stress. The results from the ChIP-seq and phenotypic and transcriptome comparison between the bzip68 mutant and wildtype indicate that bZIP68 normally suppresses expression of stress tolerance genes and promotes expression of growth-related genes, whereas its inactivation enhances stress tolerance but suppresses growth. bZIP68 might balance stress tolerance with growth through the extent of its oxidative inactivation according to the environment.

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Characterization of an Entamoeba histolytica High-Mobility-Group Box Protein Induced during Intestinal Infection

Project description:The unicellular eukaryote Entamoeba histolytica is a human parasite that causes amebic dysentery and liver abscess. A genome-wide analysis of gene expression modulated by intestinal colonization and invasion identified an up-regulated transcript that encoded a putative high-mobility-group box (HMGB) protein, EhHMGB1. We tested if EhHMGB1 encoded a functional HMGB protein, and determined its role in control of parasite gene expression. Recombinant EhHMGB1 was able to bend DNA in vitro, a characteristic of HMGB proteins. Core conserved residues required for DNA bending activity in other HMGB proteins were demonstrated by mutational analysis to be essential for EhHMGB1 activity. EhHMGB1 was also able to enhance the binding of human p53 to its cognate DNA sequence in vitro, which is expected for an HMGB1 protein. Confocal microscopy, using antibodies against the recombinant protein, confirmed its nuclear localization. Overexpression of EhHMGB1 in HM1: IMSS trophozoites led to modulation of 33 transcripts involved in a variety of cellular functions. Of these, twenty were also modulated at either day one or day 29 in the mouse model of intestinal amebiasis. Notably, four transcripts with known roles in virulence, including two encoding Gal/GalNAc lectin light chains, were modulated in response to EhHMGB1 overexpression. We concluded that EhHMGB1 was a bona fide HMGB protein with the capacity to recapitulate part of the modulation of parasite gene expression seen during adaptation to the host intestine. 6 samples for two groups: 3M and Gir. Each groups contain 3 samples

2008-11-19 | E-GEOD-12204 | biostudies-arrayexpress

Characterization of an Entamoeba histolytica High-Mobility-Group Box Protein Induced during Intestinal Infection

2008-11-20 | GSE12204 | GEO