Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profiling of U87 and U373 after 72 hours treatment of 1U/ml pegylated arginase I BCT-100.

ABSTRACT: Transcriptional profiling of U87 and U373 after 72 hours treatment of 1U/ml pegylated arginase I BCT-100.

PROVIDER: PRJNA977709 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transcriptional profiling of U87 and U373 after 72 hours treatment of 1U/ml pegylated arginase I BCT-100.

Project description:In this study the effect of pegylated arginasse I on global gene expression in U87 and U373 were examined. Both cells were cultured in complete medium with PBS control or 1U/ml pegylated arginase I (BCT-100) for 72 hours. Comparative transcriptome analysis of both cell lines in PBS control versus BCT-100 treatment were performed.

2025-05-19 | GSE233759 | GEO

RNA-seq Analysis of U87: Ctrl, U87:EGFR, U87:EGFRvIII, U87:EGFR+EGFRvIII and U87:EGFR+EGFRvIII KRAS KO

Project description:RNA-seq Analysis of U87: Ctrl, U87:EGFR, U87:EGFRvIII, U87:EGFR+EGFRvIII and U87:EGFR+EGFRvIII KRAS KO

| PRJNA483397 | ENA

Migration and invasion of 5 glioblastoma cell lines

Project description:Glioblastoma cells are characterized by a highly invasive behavior whose mechanisms are not yet understood. Using the wound healing and Boyden chamber assays we compared in the present study the migration and invasion abilities of 5 glioblastoma cell lines (DK-MG, GaMG, U87-MG, U373-MG, SNB19) differing in p53 and PTEN status. We also analyzed by Western blotting the expression of PTEN, p53, mTOR and several other marker proteins involved in cell adhesion, migration and invasion. Among 5 cell lines, GaMG cells exhibited the fastest rate of wound closure, whereas U87-MG cells showed the most rapid chemotactic migration in the Boyden chamber assay. In DK-MG and GaMG cells, F-actin mainly occurred in the numerous stress fibers spanning the cytoplasm, whereas U87-MG, U373-MG and SNB19 cells preferentially expressed F-actin in filopodia and lamellipodia. Moreover, the two glioblastoma lines mutated in both p53 and PTEN genes (U373-MG and SNB19) were found to exhibit the fastest invasion rates through the Matrigel matrix.

2014-09-05 | GSE53124 | GEO

Expression data from transfection of SW1783 and U87 glioma cells with miR-376a* for 24 and 72 hours

Project description:This SuperSeries is composed of the following subset Series: GSE34454: Expression data from transfection of SW1783 glioma cells with microRNA-376a* for 24 hours GSE34455: Expression data from transfection of U87 glioma cells with miR-376a* for 24 hours GSE34456: Expression data from transfection of U87 glioma cells with miR-376a* for 72 hours Refer to individual Series

2012-05-31 | E-GEOD-34482 | biostudies-arrayexpress

Expression data from U87 glioma cells and U87-2M1 glioma cells

Project description:An experimental lung metastasis assay was used to derive an invasive subline of U87 that is metastatic in mice. We used microarray analyses to find out over-represented gene ontologies that can explain the observed enhanced invasiveness of U87-2M1 cells. Early passage U87-2M1 cells and parental U87 glioma cells from ATCC were selected for RNA extraction and hybridization on microarray

2012-12-31 | E-GEOD-35169 | biostudies-arrayexpress

Expression data from transfection of U87 glioma cells with miR-376a* for 72 hours

Project description:Several biological pathways can be under the regulation of miRNAs. These pathways can be indentified by the enforced expression of a miRNA and analysing the expression data for enrichment of specific pathways represented among the genes differentially expressed upon miRNA overexpression. We transfected unedited miR-376a* (376a*A) and edited miR-376a* (376a*G) into U87 cells to identify differentially regulated transcripts. List of differentially expressed genes were subject to pathway enrichment analysis to identify specific biological processes regulated by each of miR-376a*A and miR-376a*G. U87 cells (100,000) were transfected with 50 nM of miR-376a*A, miR-376a*G or miR-control. Following incubation for 72 hours, total RNA was isolated from transfected cells. For each (miR-376a*A or miR-376a*G), genes differentially expressed upon miRNA transfection were determined based on comparison with miR-control-transfected samples. Biological triplicates were used for each sample type.

2012-05-31 | E-GEOD-34456 | biostudies-arrayexpress

RNA-seq Analysis of U87: Ctrl, U87:EGFR, U87:EGFRvIII, U87:EGFR+EGFRvIII and U87:EGFR+EGFRvIII KRAS KO

Project description:We found that U87 cells expressing EGFR and EGFRvIII upregulated a large group of cytokines. The upregulation of many of these cytokines was dependent on KRAS.

2018-11-01 | GSE117838 | GEO

Expression data from U87 glioma cells and U87-2M1 glioma cells

2012-12-31 | GSE35169 | GEO

ROCK1 KD in U87 cells

Project description:ROCK1 KD in U87 cells Samples were collected at 24h or 48h after siRNA ROCK1 transfection or control siRNA (NSC) in U87 cells

2016-01-28 | GSE43815 | GEO

RhoE KD in U87 cells

Project description:RhoE KD in U87 cells Samples were collected at 24h or 48h after siRNA RHoE transfection or control siRNA (NSC) in U87 cells

2016-01-28 | GSE43812 | GEO