Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of Usp17l overexpression on 2c gene in mouse embryonic stem cells

ABSTRACT: Effect of Usp17l overexpression on 2c gene in mouse embryonic stem cells

PROVIDER: PRJNA981134 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24871401_1.fastq.gz	Fastqsanger.gz
	SRR24871401_2.fastq.gz	Fastqsanger.gz
	SRR24871402_1.fastq.gz	Fastqsanger.gz
	SRR24871402_2.fastq.gz	Fastqsanger.gz
	SRR24871403_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Effect of Usp17l overexpression on 2c gene in mouse embryonic stem cells

Project description:In order to study the regulation of Usp17l on 2c gene, we overexpressed Usp17le We then performed gene expression profiling with the data obtained from the RNA-seq 48h after Usp17le overexpression .

2023-12-18 | GSE234366 | GEO

Effect of Usp17l knockdown on 2c gene in mouse embryonic stem cells (RNA-Seq)

Project description:In order to study the regulation of Usp17l on 2c gene, we established Usp17l knockdown cell lines KD4 and KD9. shRNA targets the common regions of the five subtypes of Usp17l We then performed gene expression profiling with the data obtained from the RNA-seq of the 7th generation cells.

2025-01-16 | GSE223067 | GEO

Usp17l affects 2c gene transcription by regulating H2AK119ub

Project description:We found that H2AK119ub could regulate 2 cell genes, Usp17l could also activate 2c gene, and Usp17l could remove ubiquitination modification of H2AK119ub. Therefore, in order to determine whether Usp17l affects 2c gene transcription by regulating H2AK119ub, we knockdown Usp17l. The enrichment of H2AK119ub/H3K27me3 was detected by CUT&Tag . We also sorted out Zscan4 positive and negative cells, and detected the concentration of H3K27me3 by cut-tag

2025-02-19 | GSE224711 | GEO

Usp17l can regulate the expression of 2C gene through Dux in mouse embryonic stem cells

Project description:In order to study Usp17l can regulate 2C gene expression through Dux, we overexpressed Usp17le and knockdown Dux.Then we performed gene expression profiling with the data obtained from the RNA-seq.

2025-06-05 | GSE277982 | GEO

Effect of Usp17l knockdown on 2c gene in mouse embryonic stem cells (RNA-Seq)

Project description:Effect of Usp17l knockdown on 2c gene in mouse embryonic stem cells (RNA-Seq)

| PRJNA924793 | ENA

Usp17l affects 2c gene transcription by regulating H2AK119ub

Project description:Usp17l affects 2c gene transcription by regulating H2AK119ub

| PRJNA932257 | ENA

Usp17l can regulate the expression of 2C gene through Dux in mouse embryonic stem cells

Project description:Usp17l can regulate the expression of 2C gene through Dux in mouse embryonic stem cells

| PRJNA1165157 | ENA

GADD45 promotes locus specific DNA demethylation and 2C cycling in embryonic stem cells

Project description:Mouse embryonic stem cell cultures contain a rare cell population of "2C-like" cells resembling 2-cell embryos, the key stage of zygotic genome activation (ZGA). Little is known about positive regulators of the 2C-like state and 2-cell stage embryos. We found that GADD45 (growth arrest and DNA damage 45) proteins, regulators of TET mediated DNA demethylation, promote both states. This SuperSeries is composed of the SubSeries listed below.

2019-05-31 | GSE127720 | GEO

Ythdf2 Suppresses the 2C-like State in Mouse Embryonic Stem Cells via Cooperation with Cnot1 [RNA-seq_siCNOT1]

Project description:Mouse embryonic stem cells (ESCs) consist of a rare population of heterogeneous cells which express 2-cell-stage-specific transcripts and are referred to as 2-cell-like cells (2CLCs). Accumulating evidence has demonstrated that transcription factors and epigenetic modifications exert crucial functions in the transition of ESCs to 2CLCs. However, the roles of RNA modification in the regulation of 2C-like state remain elusive. Using Dux-induced 2CLCs system, we examine N6-methyladenosine (m6A) modification landscape in transcriptome-wide, and find that m6A is dynamically regulated during Dux-driven 2C-like reprogramming. Intriguingly, many of 2C-specific transcripts are highly methylated, including Dux and Zscan4 cluster genes. We further identify the m6A reader protein Ythdf2 as a critical regulator of 2C-like state. Depletion of Ythdf2 facilitates robust expressions of 2C transcripts and the transition of ESCs to 2CLCs. Mechanically, Ythdf2 binds to the Dux/Zscan4 transcripts and promotes their degradation through recruiting the key component of RNA deadenylase complex, Cnot1. Consistent with the phenotype of Ythdf2 deficiency, silencing of Cnot1 induces the 2C gene expressions and the transition of ESCs into the 2C-like state. Collectively, our findings reveal novel insights into the epitranscriptomic regulation of the 2C-like state in mouse ESCs.

2025-03-01 | GSE281591 | GEO

Ythdf2 Suppresses the 2C-like State in Mouse Embryonic Stem Cells via Cooperation with Cnot1 [RNA-seq_Ythdf2rescue]

2025-03-01 | GSE281590 | GEO