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Red blood cells-heme-exposure and tumor-associated macrophage identity

ABSTRACT: Red blood cells-heme-exposure and tumor-associated macrophage identity

PROVIDER: PRJNA993993 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25243572.fastq.gz	Fastqsanger.gz
	SRR25243573.fastq.gz	Fastqsanger.gz

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Investigation of Bach2 function by heme-iron homeostasis in red-pulp macrophage.

Project description:Macrophages are central in regulating iron homeostasis. Transcription repressor Bach2 regulates by heme. Here we investigated the relationship between heme-regulated Bach2 and macrophage in spleen. We found that gene expression were not many change between WT and Bach2 knock out mice in red-pulp macrophage.Our results suggest that the function of the red-pulp macrophage is not dependent on according to expression of Bach2.

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Red blood cells-heme-exposure and tumor-associated macrophage identity

Project description:To study TAM phenotypes in hemorrhagic tumor tissues, we treated GFP-MC3 tumor-bearing mice with a single injection of immunotherapeutic anti-CD40 antibody on day 7 after tumor cell injection. Three days later, the tumors were collected for histology and genome-wide spatial analysis of mRNA expression. In both tumor samples, H&E histology visualized extensive tumor necrosis with RBCs extravasated into the necrotic areas of the tumors. Spatial gene-expression analysis allowed us to perform more detailed phenotyping, clearly discriminating zones of remaining viable cancer surrounded by hemorrhagic necrosis as evidenced by the enrichment of RBC-derived RNA (Hbb-bs). In these hemorrhagic regions, we found a Cd68 signal, confirming macrophage infiltration, superimposed by a strong signal for Hmox1 and Arg1. Like CD163 and Spp1 in human cancers, Arg1 is the archetypal marker gene for pro-cancerous and immunosuppressive TAMs in the mouse.

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