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In eukaryotic translation, the termination and recycling phases are linked to subsequent initiation by persistence of several factors. These comprise the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP-binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling f...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-10-16 | PXD020849 | Pride
Ribosome profiling of ABCE1-knockout cells
RNA-seq of ABCE1-knockout cells
Ribosome profiling under ABCE1 depletion to study aberrant translation termination
We report the effect of siRNA-mediated ABCE1 KD in HeLa cells, using a modified ribosome profiling protocol adapted from Ingolia et al., 2011 in order to specifically investigate ABCE1 role on translation termination
ORGANISM(S): Homo sapiens 
2020-06-30 | GSE143301 | GEO
Nonsense-mediated decay (NMD) is a pathway that degrades messenger RNAs containing premature termination codons. Here, a genome-wide screen for NMD factors uncovered an unexpected mechanism that broadly governs 3ʹ untranslated region (UTR)-directed regulation. The screen revealed that NMD requires l...
ORGANISM(S): Homo sapiens 
2020-07-14 | GSE144163 | GEO
Nonsense-mediated decay (NMD) is a pathway that degrades messenger RNAs containing premature termination codons. Here, a genome-wide screen for NMD factors uncovered an unexpected mechanism that broadly governs 3ʹ untranslated region (UTR)-directed regulation. The screen revealed that NMD requires l...
ORGANISM(S): Homo sapiens 
2020-07-14 | GSE144164 | GEO
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