Human ABCE1 exhibits temperature-dependent heterologous co-functionality in S. cerevisiae.
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ABSTRACT:
SUBMITTER: Wada M
PROVIDER: S-EPMC9527587 | biostudies-literature | 2022 Oct
REPOSITORIES: biostudies-literature
Publications
Human ABCE1 exhibits temperature-dependent heterologous co-functionality in S. cerevisiae.
FEBS open bio 20220719 10
ABCE1 protein (Rli1 in Saccharomyces cerevisiae) is a unique ribosome recycling factor that is composed of an N-terminal FeS cluster domain and two ATPase domains. Here, we report that heterologous expression of human ABCE1 in S. cerevisiae is unable to complement conditional knockout of ABCE1 (Rli1), at a typical experimental temperature of 30 °C. However, low but significant growth was observed at high temperature, 37 °C. Considering the close interaction of ABCE1 with translation factors and ...[more]
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