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TranscriptomicsImpact of oxidative stress on ascorbate biosynthesis in Chlamydomonas via regulation of the VTC2 gene encoding a GDP-l-galactose phosphorylase
The l-galactose (Smirnoff-Wheeler) pathway represents the major route to l-ascorbic acid (vitamin C) biosynthesis in plants. Arabidopsis thaliana VTC2 and its paralogue VTC5 function as GDP-l-galactose phosphorylases converting GDP-l-galactose to l-galactose-1-P, thus catalyzing the first committed ...
ORGANISM(S): Chlamydomonas reinhardtii 
2012-05-02 | E-GEOD-34826 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis wild-type, thylakoidal ascorbate peroxidase overexpressor (over tAPX, line 14/2), flu mutant and flu plants overexpressing thylakoidal ascorbate peroxidase
We used the flu mutant of Arabidopsis and a transgenic line that overexpresses the thylakoid-bound ascorbate peroxidase (tAPX) to address the interactions between different reactive oxygen species (ROS) signaling pathways. The conditional flu mutant of Arabidopsis accumulates excess protochlorophyll...
ORGANISM(S): Arabidopsis thaliana 
2008-04-05 | E-GEOD-10812 | biostudies-arrayexpress
ProteomicsAPX2, the ascorbate peroxidase-related protein, regulates the levels of plastocyanin in Chlamydomonas
The function of ascorbate peroxidase-related (APX-R) proteins, present in all green photosynthetic eukaryotes, remains unclear. This study focuses on APX-R from Chlamydomonas reinhardtii, namely ascorbate peroxidase 2 (APX2). We showed that apx2 mutants exhibited a faster oxidation of the PSI primar...
ORGANISM(S): Chlamydomonas reinhardtii 
2024-01-29 | PXD046605 | Pride
Chlamydomonas Apx2 Plastocyanin Cytochrome c6 Copper-deficiency P700 
ProteomicsAscorbate peroxidase (APEX2) -mediated proximity-based proteomics for HTT protein interactions
Huntington’s Disease (HD), a progressive neurodegenerative disorder with no disease-modifying therapies, is caused by a CAG repeat expansion in the HD gene encoding polyglutamine-expanded huntingtin (HTT) protein. Mechanisms of HD cellular pathogenesis and cellular functions of the normal and mutan...
ORGANISM(S): Homo sapiens (Human) 
2026-03-16 | PXD059663 | Pride
Huntington’s disease Protein interactions Huntingtin Dna damage Neurodegeneration 
ProteomicsAn in situ ascorbate peroxidase 2-based labeling method and its application in detecting exosome proteins released from mouse proximal tubule cells under oxidative stress
Here, we developed an in situ labeling approach to identify exosome-loaded proteins, which bypasses the traditional exosome isolation steps. In this method, an engineered ascorbate peroxidase targeting to endosomes-destined exosomes was used to label proteins loaded inside exosomes released from kid...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-12 | PXD025852 | Pride
Apex2 Kidney Mouse Oxidative stress Exosomes Lc-ms/ms 
UnknownEngineering ascorbate peroxidase activity into cytochrome c peroxidase.
Not available
S-EPMC2770236 | biostudies-literature
TranscriptomicsArabidopsis thaliana Transcriptome under Salt Stress
CuZn-superoxide dismutase (CuZn-SOD) and ascorbate peroxidase (APX) constitute first line of defence against oxidative stress. In the present study, PaSOD and RaAPX genes from Potentilla atrosanguinea and Rheum australe, respectively were overexpressed individually as well as in combination in Arabi...
ORGANISM(S): Arabidopsis thaliana 
2014-10-06 | E-MTAB-4434 | biostudies-arrayexpress
TranscriptomicsGene expression between dry and imbibed seeds in vitamin C defective (vtc) mutants and wild-type Arabidopsis thaliana
In the dry state, orthodox seeds have little antioxidant capacity, relying on reduced glutathione and tocochromanols for antioxidant defences rather than ascorbate or ascorbate peroxidase (APX). Ascorbate is synthesised de novo within a few hours of imbibition resulting in rapid increases in the asc...
ORGANISM(S): Arabidopsis thaliana 
2017-07-03 | E-MTAB-5103 | biostudies-arrayexpress
ProteomicsDevelopment and Comparative Evaluation of Endolysosomal Proximity Labeling-based Proteomic Methods in Human iPSC-derived Neurons
Proximity-based in situ labeling techniques offer a unique way to capture both stable and transient protein-protein and protein-organelle interactions. Combining this technology with mass spectrometry (MS)-based proteomics allows us to obtain snapshots of molecular microenvironments with nanometer r...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-10-10 | MSV000086260 | MassIVE
TranscriptomicsExpression data from thylakoidal ascorbate peroxidase overexpressor Arabidopsis thaliana (Col) rosette leaves
We used the flu mutant of Arabidopsis and a transgenic line that overexpresses the thylakoid-bound ascorbate peroxidase (tAPX) to address the interactions between different reactive oxygen species (ROS) signaling pathways. The conditional flu mutant of Arabidopsis accumulates excess protochlorophyll...
ORGANISM(S): Arabidopsis thaliana 
2008-03-13 | GSE10812 | GEO
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