To investigate the effect of ATP2 knockdown on the protein expression of Candida albicans, we performed proteomic assays for atp2Δ/Δ and wild type strains. A total of 4000.0 proteins were identified in this study, of which 3652.0 proteins contained quantitative information. If a 1.5-fold differentia...
Broad host range species in specialised pathogens should be treated with suspicion – A case study in Entyloma reveals high, host-correlated diversity in pathogens infecting Ranunculus
We performed subcellular fractionation of the polysomes associated with the mitochondrial surface (mitopolysomes) in wild type or Tma108 deleted yeast strains and measured the mRNA enrichment in these fractions by competitive hybridization on microarrays against the corresponding total RNAs. This wa...
We performed RIP (RNA-IP experiments) in the yeast Saccharomyces cerevisiae, immunoprecipitating either Tma108, Scp160, Tma46 or Rpl16. The RNAs present in the IPs were analyzed by microarray competitive hybridization against total RNAs (Input). Our goal was to identify the RNAs which are targeted,...
