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TranscriptomicsGenomic Library Enrichment for n-Butanol tolerance in E. coli. Samples vs Reference
Genomic Library Enrichment to determine the n-Butanol Tolerant related genes in E. coli. The samples involves a series of batch transfers to increasing concentrations of n-butanol and controls (always grew in absence of the solvent) Two technical replicas. Samples 1, 3, 5, and 7 are the serial tra...
ORGANISM(S): Escherichia coli 
2012-09-19 | E-GEOD-26223 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of isolated Escherichia coli mutants using VERT for the study of n-butanol tolerance
Transcriptome analysis of isolated mutants using the method Visualizing Evolution in Real-Time (VERT) for the study of n-butanol tolerance. The samples were isolated from an evolution experiment picking samples at different times based in the evolution dynamics obtained with VERT. Mutants were gro...
ORGANISM(S): Escherichia coli K-12 
2012-03-30 | E-GEOD-30005 | biostudies-arrayexpress
MetabolomicsAdaptive Evolution Reveals Mechanisms of Isobutanol Tolerance in Zymomonas mobilis

Isobutanol production has been demonstrated in E. coli and Z. mobilis using heterologous pathways, with titers reaching up to 60% of the theoretical yield under optimized conditions. However, these results often depend on impractical setups, such as continuous product extraction, oxygenation, or ...

2026-03-18 | MTBLS14081 | MetaboLights
ProteomicsClostridium cellulovorans proteomic responses to butanol stress
Combination of butanol-hyperproducing and hypertolerant phenotypes is essential for developing microbial strains suitable for industrial production of bio-butanol, i.e. one of most promising liquid biofuels. Clostridium cellulovorans is among the microbial strains with the highest potential for dire...
ORGANISM(S): Clostridium cellulovorans 
2021-08-13 | PXD024183 | Pride
Atpase Rex Stringent response Hfq Heat shock proteins 
TranscriptomicsButanol Challenge of L. brevis
The presence of anti-microbial phenolic compounds, such as the model compound ferulic acid, in biomass hydrolysates poses significant challenges to the widespread use of biomass in conjunction with whole cell biocatalysis or fermentation. Biofuel toxicity must also be overcome to allow for efficient...
ORGANISM(S): Lactobacillus brevis 
2011-12-31 | E-GEOD-24944 | biostudies-arrayexpress
TranscriptomicsFunctional Genomic Study of Exogenous n-Butanol Stress in Escherichia coli
n-Butanol has been proposed as an alternative biofuel to ethanol, and both Escherichia coli and Saccharomyces cerevisiae have been engineered to produce it. Unfortunately, n-butanol is more toxic than ethanol to these organisms. To understand the basis for its toxicity, cell wide studies were cond...
ORGANISM(S): Escherichia coli 
2010-05-16 | E-GEOD-16973 | biostudies-arrayexpress
ProteomicsProteomics of Pseudomonas putida BIRD-1
Understanding Butanol Tolerance and Assimilation in Pseudomonas putida BIRD-1, analysis of soluble proteins
ORGANISM(S): Pseudomonas putida (strain BIRD-1) 
2018-10-24 | PXD002679 | Pride
Shotgun proteomics  butanol tolerance  butanol metabolism  ms/ms 
ProteomicsProteomics of Pseudomonas putida BIRD-1
Understanding Butanol Tolerance and Assimilation in Pseudomonas putida BIRD-1, analysis of proteins associated to membranes
ORGANISM(S): Pseudomonas putida (strain BIRD-1) 
2018-10-24 | PXD002655 | Pride
Shotgun proteomics  butanol tolerance  butanol metabolism  ms/ms 
TranscriptomicsDNA Microarray of Global Transcription Factor Mutant Reveals Membrane-Related Proteins Involved in n-Butanol Tolerance in Escherichia coli
We successfully isolated an E. coli strain harboring rpoD mutant B8 with 2% (v/v) butanol tolerance using global transcriptional machinery engineering approach. DNA microarrays were employed to assess the transcriptome profile of n-butanol tolerance strain B8 and control strain E. coli JM109. The go...
ORGANISM(S): Escherichia coli O157:H7 str. Sakai Escherichia coli CFT073 Escherichia coli Escherichia coli O157:H7 str. EDL933 Escherichia coli str. K-12 substr. MG1655 
2016-05-10 | GSE79305 | GEO
Proteomicsquantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7
Clostridium sp. strain CT7 is a new emerging microbial cell factory with high butanol ratio owing to the non-traditional butanol fermentation mode with uncoupled acetone and 1,3-propanediol formation. Significant change of products profile was shown in glycerol- and glucose-fed strain CT7, especiall...
ORGANISM(S): uncultured Clostridium sp. 
2021-09-08 | PXD016848 | Pride
Itraq  proteomic analysis  glycerol  butanol  clostridium 
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