Sort   by:  
 Page size 
MetabolomicsMetabolic Drug Response Phenotyping in Colorectal Cancer Organoids by LC-QTOF-MS
As metabolic rewiring is crucial for cancer cell proliferation, metabolic phenotyping of patient-derived organoids is desirable to identify drug-induced changes and trace metabolic vulnerabilities of tumor subtypes. We established a novel protocol for metabolomic and lipidomic profiling of colorecta...
2021-10-12 | MTBLS2130 | MetaboLights
MetabolomicsA KRAS-triggered lactylation cascade forms the basis of chemotherapy resistance in colorectal cancer
KRAS-mutant colorectal cancer (KRASmut CRC) exhibits metabolic plasticity and chemoresistance, but the underlying mechanisms remain incompletely understood. Here, we show in CRC cells and organoids that KRAS-driven lactate accumulation promotes MCT1 K224 lactylation, which stabilizes mitochondrial M...
2026-10-16 | MTBLS16030 | MetaboLights
MetabolomicsA KRAS-triggered lactylation cascade forms the basis of chemotherapy resistance in colorectal cancer

KRAS-mutant colorectal cancer (KRASmut CRC) exhibits metabolic plasticity and chemoresistance, but the underlying mechanisms remain incompletely understood. Here, we show in CRC cells and organoids that KRAS-driven lactate accumulation promotes MCT1 K224 lactylation, which stabilizes mitochondria...

2026-10-16 | MTBLS16031 | MetaboLights
MetabolomicsLactate controls cancer stemness and plasticity through epigenetic regulation.

Tumours arise from uncontrolled cell proliferation driven by mutations in genes that regulate stem cell renewal and differentiation. Intestinal tumours, however, retain some hierarchical organization, maintaining both cancer stem cells (CSCs) and cancer differentiated cells (CDCs). This heterogen...

2025-05-28 | MTBLS11849 | MetaboLights
MetabolomicsA KRAS-triggered lactylation cascade forms the basis of chemotherapy resistance in colorectal cancer

KRAS-mutant colorectal cancer (KRASmut CRC) exhibits metabolic plasticity and chemoresistance, but the underlying mechanisms remain incompletely understood. Here, we show in CRC cells and organoids that KRAS-driven lactate accumulation promotes MCT1 K224 lactylation, which stabilizes mitochondria...

2026-10-16 | MTBLS16037 | MetaboLights
MetabolomicsA KRAS-triggered lactylation cascade forms the basis of chemotherapy resistance in colorectal cancer
KRAS-mutant colorectal cancer (KRASmut CRC) exhibits metabolic plasticity and chemoresistance, but the underlying mechanisms remain incompletely understood. Here, we show in CRC cells and organoids that KRAS-driven lactate accumulation promotes MCT1 K224 lactylation, which stabilizes mitochondrial M...
2026-10-16 | MTBLS16034 | MetaboLights
TranscriptomicsAnalysis of human enteroids and colonoids (3D organoid models from small intestine and colon) exposed to chemotherapy agent 5-fluorouracil (5FU) in a dose-repeated exposure across three doses (10,100 and 1000uM) and sampled at three points (24, 48 and 72h) since initial exposure. Organoids were obtained from healthy human tissues.
ADRs (adverse drug reactions) are one of the main reasons for treatment discontinuation or alterations in dose regimens in clinical settings. Chemotherapy-induced ADRs are common, especially gastrointestinal-induced ones. Within TransQST (transqst.org), one of the main goal is to improve the in vitr...
ORGANISM(S): Homo sapiens 
2021-09-09 | E-MTAB-9134 | biostudies-arrayexpress
ProteomicsPersonalized proteome profiles of healthy and tumor human colon organoids reveal both individual diversity and basic features of colorectal cancer.
We performed quantitative mass spectrometry based proteomic analysis and comparative transcriptomics analysis of 7 individual patient-derived human colorectal tumor and healthy organoids, the latter acquired from adjacent healthy tissue.
ORGANISM(S): Homo sapiens (Human) 
2017-01-16 | PXD004149 | Pride
Proteomics Organoids Colon cancer 
TranscriptomicsRNA-seq of BRAF-V600E_mutated and BRAF-V600E_corrected colon cancer organoids_advanced starvation
We aimed to investigate transcriptional changes in human colon cancer organoids with the BRAF-V600E mutation and in human colon cancer organoids in which the BRAF-V600E mutation was corrected by means of CRISPR genome editing. RNAseq was performed at USEQ at the UMC Utrecht (The Netherlands). Simila...
ORGANISM(S): Homo sapiens 
2024-08-23 | E-MTAB-14298 | biostudies-arrayexpress
TranscriptomicsRNA-seq of BRAF-V600E_mutated and BRAF-V600E_corrected colon cancer organoids
We aimed to investigate transcriptional changes in human colon cancer organoids with the BRAF-V600E mutation and in human colon cancer organoids in which the BRAF-V600E mutation was corrected by means of CRISPR genome editing. RNAseq was performed at USEQ at the UMC Utrecht (The Netherlands).
ORGANISM(S): Homo sapiens 
2024-02-21 | E-MTAB-13806 | biostudies-arrayexpress
Sort   by:  
 Page size