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ProteomicsPeptide mass fingerprinting to investigate if histidine was incorporated as residue 136 in DHFR variants
SDS-PAGE gel bands of DHFR variants (produced using modified versions of the PURE express in vitro protein synthesis system) were analyzed via peptide mass fingerprinting (PMF). The goal was to detect which amino acids were incorporated at position 136 in DHFR.
ORGANISM(S): Escherichia coli 
2024-10-29 | PXD051338 | Pride
Lc-ms/ms Dhfr variants Pmf 
ProteomicsMethotrexate-based PROTACs as DHFR chemical probes
Methotrexate (MTX) is a potent inhibitor of dihydrofolate reductase (DHFR), where is it used as both an antineoplastic and an immunosuppressant. Mechanisms of MTX resistance in cancers include MTX polyglutamylation and upregulation of DHFR. A series of MTX-based PROteolysis TArgeting Chimeras (PROTA...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD030140 | Pride
Dhfr Targeted protein degradation Methotrexate Chemical probe Protac One carbon metabolism 
GenomicsBlocking protein quality degradation leads to structural stabilization of DHFR indel variants
Blocking protein quality degradation leads to structural stabilization of DHFR indel variants
PRJNA1289146 | ENA
OtherBlocking protein quality degradation leads to structural stabilization of DHFR indel variants
Gene variants leading to insertions or deletions of amino acid residues (indels) often have detrimental consequences for the folding of the encoded protein. Yet at some positions indels are tolerated or only result in partial unfolding. Typically unfolded proteins are targeted for protein quality co...
ORGANISM(S): Homo sapiens 
2025-12-22 | GSE302176 | GEO
MetabolomicsMetabolic response to point mutations reveals principles of modulation of in vivo enzyme activity and phenotype
The relationship between sequence variation and phenotype is poorly understood. Here, we use metabolomic analysis to elucidate the molecular mechanism underlying the filamentous phenotype of E. coli strains that carry destabilizing mutations in dihydrofolate reductase (DHFR). We find that partial lo...
2021-06-30 | MTBLS2795 | MetaboLights
ProteomicsPyrimethamine and a potent analogue WCDD115 inhibit NRF2 by suppressing DHFR and one-carbon metabolism
Nuclear factor erythroid 2-related factor 2 (NFE2L2/NRF2) is a critical mediator of the cellular oxidative stress response. Aberrant activation of NRF2 is common in many lung and upper aerodigestive cancers, where it promotes tumor progression and confers resistance to chemotherapy, radiation therap...
ORGANISM(S): Homo sapiens (Human) 
2025-10-13 | PXD059011 | Pride
Human Dhfr Cancer Nrf2 
TranscriptomicsMicroarray Profiling of Messenger RNAs Associated with DHFR Knock-down and DHFR2 Knockout in HepG2 cell line
Dihydrofolate reductase activity is essential for the maintenance of One Carbon Metabolism, as it provides the pathway with tetrahydrofolate (the biologically active form of folate). Most of this cellular activity is due to DHFR. Its paralogue DHFR2 was thought to be responsible for mitochondrial di...
ORGANISM(S): Homo sapiens 
2024-02-01 | E-MTAB-12958 | biostudies-arrayexpress
UnknownMethotrexate-based PROTACs as DHFR-specific chemical probes.
Not available
S-EPMC10922102 | biostudies-literature
GenomicsEscherichia coli strain:ER2566
E. Coli DHFR Deep Mutational Scanning
PRJNA590072 | ENA
Genomics
Laboratory evolution of dihydrofolate reductase (DHFR)
PRJNA923701 | ENA
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