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ProteomicsDifferential alkylation of PHLPP2
To determine the accessibility of cysteines in purified PHLPP2, we assessed their reactivity with iodoacetamide under native conditions. After denaturing with urea and reducing potential disulfide bridges with TCEP, the remaining free cysteines were modified with methyl methanethiosulfonate. This 2-...
ORGANISM(S): Homo sapiens (Human) 
2025-03-13 | PXD052551 | Pride
Differential alkylation Free thiols 
ProteomicsIdentification of cysteine redox states in MTP from PDI-deficient mouse liver
Cysteine redox states in MTP from PDI-deficient mouse liver were determined using differential cysteine alkylation and PRM-MS analysis.
ORGANISM(S): Mus musculus (Mouse) 
2025-01-16 | PXD055650 | Pride
Mtp Differential cysteine alkylation Prm-ms Redox state of cysteines 
ProteomicsOxSWATH applied to the redox characterization of secretomes under oxidative stress conditions
Most of the redox proteomics strategies are focused on the identification and relative quantification of cysteine oxidation changes without considering the variation in the total levels of the proteins. However, the regulation of protein synthesis and protein degradation are also associated with man...
ORGANISM(S): Homo sapiens (Human) 
2019-02-15 | PXD006802 | Pride
Redoxomics  cysteine oxidation  swath-ms  differential alkylation  secretome  dj-1 
ProteomicsOxSWATH applied to in-vitro the characterization of redox state of recombinant DJ-1 proteins
Most of the redox proteomics strategies are focused on the identification and relative quantification of cysteine oxidation changes without considering the variation in the total levels of the proteins. However, the regulation of protein synthesis and protein degradation are also associated with man...
ORGANISM(S): Homo sapiens (Human) 
2019-02-16 | PXD006803 | Pride
Redoxomics  cysteine oxidation  swath-ms  differential alkylation  recombinant dj-1 
UnknownIdentification of reduction-susceptible disulfide bonds in transferrin by differential alkylation using O(16)/O(18) labeled iodoacetic acid.
Not available
S-EPMC4401651 | biostudies-literature
ProteomicsQuantification of sulfenic acid on model protein
Aldolase contains eight free cysteine residues without disulfide formation in its native state, which makes it a suitable model to manipulate the oxidative modifications on cysteine. H2O2 was chosen to treat aldolase since the principle product between which is sulfenic acid.To assess the reduction ...
ORGANISM(S): Lagomorpha Mammalia Gallus gallus (Chicken) 
2016-10-10 | PXD003348 | Pride
Lysozyme Sulfenic acid Aldolase Arsenite 
ProteomicsDifferential gene expression in Leishmania [SPP.] promastigotes. Leishmania chagasi
Leishmania chagasi is the causative agent of zoonotic visceral leishmaniasis in Brazil, being domestic and stray dogs the main reservoirs. The development of the parasite involves two stages. The promastigote is extracellular and develops within the sand fly gut. The amastigote survives inside the h...
ORGANISM(S): Leishmania chagasi 
2016-03-31 | PXD002939 | Pride
Differential gene expression Maldi Leishmania 2d-e 
TranscriptomicsRNA-sequencing using SLAM-sequencing protocol of human iPSC-derived lower motor neurons treated with BDNF for 1, 2, 6 h
SLAM-sequencing was performed on i3 LMNs following protocols adapted from Herzog et al. (2017). Three biological replicates of i3 LMNs were treated with 100 µM 4sU (Control) + labelling medium or 100 µM 4sU + BDNF (BDNF condition) labelling medium on Day 7 for 1h, 2h, and 6h. Cells were washed with ...
ORGANISM(S): Homo sapiens 
2025-05-30 | E-MTAB-14537 | biostudies-arrayexpress
UnknownSynthetic modeling of zinc thiolates: quantitative assessment of hydrogen bonding in modulating sulfur alkylation rates.
Not available
S-EPMC152984 | biostudies-literature
ProteomicsDifferential Lysis Approach Enables Selective Extraction of Taxon-Specific Proteins for Gut Metaproteomics
A differential lysis approach was developed with different strength of lysis buffers (urea and/or sodium dodecyl sulfate) and applied on human stool samples to separate different microbial species to increase the depth of metaproteomic measurements. We showed that the lysates dominating by proteins ...
ORGANISM(S): Escherichia coli 
2023-03-27 | PXD016787 | Pride
Human Lc-msms Gut microbiome 
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