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ProteomicsMapping physiological ADP-ribosylation using Activated Ion Electron Transfer Dissociation (AI-ETD)
ADP-ribosylation (ADPr) is a post-translational modification that plays pivotal roles in a wide range of cellular processes. Mass spectrometry (MS)-based analysis of ADPr under physiological conditions, without relying on genetic or chemical perturbation, has been hindered by technical limitations. ...
ORGANISM(S): Homo sapiens (Human) 
2020-09-23 | PXD017417 | Pride
Activated ion electron transfer dissociation Post-translational modification Adpr Ptm Parp Ai-etd Adp-ribosylation 
ProteomicsTop-down detection of oxidative protein footprinting by Collision-Induced Dissociation, Electron-Transfer Dissociation and Electron-Capture Dissociation
Fast photochemical oxidation of proteins (FPOP) footprinting is a structural mass spectrometry method that maps proteins by fast and irreversible chemical reactions. The position of oxidative modification reflects solvent accessibility and site reactivity and thus provides information about protein ...
ORGANISM(S): Bos taurus (Bovine) Equus caballus (Horse) 
2023-07-20 | PXD031926 | Pride
Electron-transfer dissociation Fast photochemical oxidation of proteins Top-down Collision-induced dissociation Mass spectrometry. Electron-capture dissociation 
ProteomicsConjugation site characterization of antibody–drug conjugates using electron-transfer/higher-energy collision dissociation (EThcD)
Antibody–drug conjugates (ADCs) are formed by binding of cytotoxic drugs to monoclonal antibodies (mAbs) through chemical linkers. A comprehensive evaluation of the critical quality attributes (CQAs) of ADCs is vital for drug development but remains challenging owing to ADC structural heterogeneity ...
ORGANISM(S): Homo Sapiens 
2023-02-14 | PXD040111 |
ProteomicsElectron Transfer/ Higher Energy Collisional Dissociation of Doubly Charged Peptide Ions: Identification of Labile Protein Phosphorylations
Fragmentation behaviour of labile phosphorylated peptides
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2019-05-27 | PXD012989 | Pride
Labile phosphorylations Ethcd 
ProteomicsElectron-based dissociation is needed for O-glycopeptides derived from OpeRATOR proteolysis
The recently described O-glycoprotease OpeRATOR presents exciting opportunities for O-glycoproteomics. This bacterial enzyme purified from A. muciniphila cleaves N-terminally to serine and threonine residues that are modified with (preferably asialylated) O-glycans, providing orthogonal cleavage rel...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-11-03 | PXD020077 | Pride
Glycoproteomics O-glycosites Operator Protease Electron transfer dissociation 
ProteomicsCapillary zone electrophoresis-tandem mass spectrometry with activated ion electron transfer dissociation for large-scale top-down proteomics
Capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) has been recognized as an efficient approach for top-down proteomics recently for its high-capacity separation and highly sensitive detection of proteoforms. However, the commonly used collision-based dissociation methods often ca...
ORGANISM(S): Escherichia coli 
2019-06-11 | PXD012247 | Pride
Top-down proteomics E. coli Cze-ms Ai-etd 
ProteomicsMS characterization of spidroin-1
For MS characterization of major ampullate spidroin-1 from the three species of Nephila clavipes, edulis and madagascariensis, an experimental approach was developed combining 2-DE with multiple proteolytic in-gel digestion (trypsin, chymotrypsin, pepsin, proteinase K, subtilisin and proteinase 1...
ORGANISM(S): Nephila clavipes (Golden silk orbweaver) 
2022-02-22 | PXD000057 | Pride
Major ampullate silk Nephila sp. Post-translational modifications Phosphorylation Nanolc-esi-cid/etd-ms/ms Spidroin-1 
UnknownCoupling capillary zone electrophoresis with electron transfer dissociation and activated ion electron transfer dissociation for top-down proteomics.
Not available
S-EPMC4439324 | biostudies-literature
ProteomicsCapillary Zone Electrophoresis-Electron-Capture Collision-Induced Dissociation on a Quadrupole Time-of-Flight Mass Spectrometer for Top-Down Characterization of Intact Proteins
Mass spectrometry (MS)-based denaturing top-down proteomics (dTDP) requires high-capacity separation and extensive gas-phase fragmentation of proteoforms. Herein, we coupled capillary zone electrophoresis (CZE) to electron-capture collision-induced dissociation (ECciD) on an Agilent 6545 XT quadrupo...
ORGANISM(S): Bos taurus (Bovine) Equus caballus (Horse) 
2023-01-05 | PXD030373 | Pride
Electron-capture collision-induced dissociation Top-down Capillary zone electrophoresis 
UnknownSequence analysis of peptide:oligonucleotide heteroconjugates by electron capture dissociation and electron transfer dissociation.
Not available
S-EPMC3638748 | biostudies-literature
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