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ProteomicsBioID proximity proteomics of wild-type and ΔPDZ DVL2 in HEK293 Flp-In T-REx cells
This dataset supports a study investigating how the PDZ domain of Dishevelled-2 (DVL2) specifies β-catenin-independent Wnt signalling outputs. BioID proximity labelling was performed in HEK293 Flp-In T-REx cells stably expressing BirA*-tagged wild-type DVL2 or a matched DVL2 mutant lacking the PDZ d...
ORGANISM(S): Homo sapiens (Human) 
2026-09-12 | PXD079793 | Pride
Bioid  proximity labelling  proximity proteomics  dvl2  dishevelled  pdz domain  daple  girdin  girdin-l  wnt signalling  wnt5a  primary cilia  basal body  pdz-pbm interaction  lc-ms/ms  bottom-up proteomics  data-dependent acquisition  hek293 flp-in t-rex 
TranscriptomicsRNA-Seq of WT Flp-In T-REx 293, UPF3A knockout and UPF3A overexpression cell lines in part with additional siRNA-mediated control or UPF3B knockdowns
UPF3A and UPF3B are paralogous genes in human cells that are involved in the nonsense-mediated decay (NMD) pathway. NMD is a cellular quality control mechanism that monitors mRNAs during translation. Aberrant translation due to features such as the presence of a premature stop codon downstream on an...
ORGANISM(S): Homo sapiens 
2022-08-31 | E-MTAB-10718 | biostudies-arrayexpress
TranscriptomicsGene expression profiling in HEK293 cells overexpressed lactoferrin gene that has the signal sequence (sLF) or not (ΔLF)
Human lactoferrin (LF) is a multifunctional protein involved in immunomodulation, cell growth, and differentiation. In addition to the secreted form (sLF), an alternatively spliced form (ΔLF) that lacks the signal sequence and downregulated in cancer was found. This study was carried out to identify...
ORGANISM(S): Homo sapiens 
2011-09-06 | E-GEOD-31878 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human HNF1beta mutated vs wildtype embryonic kidney cell line HEK293 to identify potential HNF1beta target genes whose activity may be deregulated in human patients
Hepatocyte nuclear factor 1beta (HNF1beta, TCF2) is a tissue-specific transcription factor whose mutation in humans leads to renal cysts, genital malformations, pancreas atrophy and maturity onset diabetes of the young (MODY5). Furthermore, HNF1beta overexpression has been observed in clear cell can...
ORGANISM(S): Homo sapiens 
2007-09-07 | E-GEOD-3308 | biostudies-arrayexpress
ProteomicsPhosphoproteome of PINK1 in HEK293 cells
triple SILAC phosphoproteomics experiment of Flp-In T-Rex HEK293 cells stably expressing either FLAG empty, PINK1-FLAG Kinase dead or PINK1-FLAG wild type were grown in ‘light’ (K0R0), ‘medium’ (K4R6) and ‘heavy’ (K8,R10) SILAC media, respectively.
ORGANISM(S): Homo sapiens (Human) 
2015-09-16 | PXD002127 | Pride
Pink1 Parkinson's disease Human Phosphorylation Phosphoproteomics Hek293 
Transcriptomicshsa-miR-92a knock down in Flp-in T-REx 293-PTH-AGO1 cells
transcriptome profiling of miR-92a inhibitor treated and control cells with the aim of measuring miR-92a influence on its mRNA targets Abstract: MicroRNAs (miRNAs) play key roles in gene regulation, but reliable bioinformatic or experimental identification of their targets remains difficult. To prov...
ORGANISM(S): Homo sapiens 
2013-04-26 | E-GEOD-46039 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human HEK293 cells overexpressing HNF4a2 wild type or mutant forms C106R or R154X
Identification of genes regulated by the transcription factor HNF4a2 Experiment Overall Design: We used microarrays to identify genes regulated by the transcription factor HNF4a2. HEK293 cell lines containing doxycyclin-inducible wilt type or mutant forms of HNF4a2m which were introduced by FRT/FLP ...
ORGANISM(S): Homo sapiens 
2008-10-18 | E-GEOD-8289 | biostudies-arrayexpress
TranscriptomicsMOV10 is a 5' to 3' RNA Helicase Contributing to UPF1 mRNA Target Degradation by Translocation Along 3' UTRs (PAR-CLIP)
RNA helicases are important regulators of gene expression that act by remodeling RNA secondary structures and as RNA-protein interactions. Here, we demonstrate that MOV10 has an ATP-dependent 5' to 3' in vitro RNA unwinding activity and determine the RNA-binding sites of MOV10 and its helicase mutan...
ORGANISM(S): Homo sapiens 
2014-04-11 | E-GEOD-48245 | biostudies-arrayexpress
GenomicsChromatin maps of human ASXL1, FOXK1 and O-GlcNAc
Polycomb group (PcG) proteins are major determinants of gene silencing and epigenetic memory in higher eukaryotes. Acting in multimeric protein complexes, these factors control structure and function of chromatin. We used a robust affinity purification mass spectrometry (AP-MS) approach to systemati...
ORGANISM(S): Homo sapiens 
2016-09-08 | E-GEOD-51673 | biostudies-arrayexpress
ProteomicsWhole cell proteome of cells lacking heme biosynthesis enzyme CPOX
Whole cell proteomics of a CRISPR/Cas9-generated HEK293 FLP/IN/T-Rex CPOX KO cell line in comparison to HEK293 FLP/IN/T-Rex WT cells and HEK293 FLP/IN/T-Rex CPOX KO cells complemented with CPOX-HA WT.
ORGANISM(S): Homo sapiens (Human) 
2026-04-16 | PXD064668 | Pride
Mitochondria  cpox  ims  heme biosynthesis 
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