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MetabolomicsLipidCreator workbench to probe the lipidomic landscape - Platelet isolation and stimulation, Targeted Phospho and Glycerolipid Profiling

Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope...

2020-04-15 | MTBLS1369 | MetaboLights
MetabolomicsLipidCreator workbench to probe the lipidomic landscape - Platelet isolation and stimulation - DIA lipid mediator validation

Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope...

2020-04-15 | MTBLS1382 | MetaboLights
MetabolomicsLipidCreator workbench to probe the lipidomic landscape - Platelet isolation and stimulation, targeted lipid mediator profiling

Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope...

2020-04-15 | MTBLS1381 | MetaboLights
MetabolomicsCommunity-integrated multi-omics facilitates screening and isolation of the organohalide dehalogenation microorganism

A variety of anthropogenic organohalide contaminants generated from industry are released into the environment, and thus cause serious pollution that endangers human health. In the present study, we investigated the microbial community composition of industrial saponification wastewater using 16S...

2022-11-21 | MTBLS3916 | MetaboLights
TranscriptomicsIsolation and in vitro expansion of human colonic stem cells
This SuperSeries is composed of the following subset Series: GSE31255: Isolation and in vitro expansion of human colonic stem cells [Expression profile] GSE31256: Isolation and in vitro expansion of human colonic stem cells [CGH] Refer to individual Series
ORGANISM(S): Homo sapiens 
2011-11-22 | E-GEOD-31257 | biostudies-arrayexpress
TranscriptomicsIsolation and in vitro expansion of human colonic stem cells [CGH]
Tissue for normal colonic stem cell isolation was obtained via colectomy from a colorectal cancer patient. It was shown that the resected tissue and the in vitro-cultured organoids grown from tissue-derived adult stem cells do not harbor chromosomal alterations. For that purpose, genomic DNA from re...
ORGANISM(S): Homo sapiens 
2011-11-22 | E-GEOD-31256 | biostudies-arrayexpress
ProteomicsProteome Alterations during Clonal Isolation of Human Pancreatic Cancer Cell Lines
Starting from an initial cell culture (Reference), three human pancreatic cancer cell lines (MIA PaCa-2, AsPC-1 and PANC-1) were either maintained by standard cell culture (Standard) or underwent clonal isolation to obtain single-cell colonies (SCC). Emerging cell pellets of five replicates per cond...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-02-23 | MSV000088904 | MassIVE
TranscriptomicsRapid, high efficiency isolation of pancreatic ß-cells
The ability to isolate pure pancreatic ß-cells would greatly aid multiple areas of diabetes research. We developed an exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional pancreatic ß-cells. By targeting the glucagon-like peptide-1 receptor, ß-cells were isolate...
ORGANISM(S): Mus musculus 
2015-09-04 | E-GEOD-64508 | biostudies-arrayexpress
ProteomicsIsolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium Synechocystis sp. PCC 6803
The FoF1 ATP synthase (ATPase) is one of the most important protein complexes in the energy metabolism, with many open questions about its assembly, regulation, and functions. The isolation of functional ATPase complexes is fundamental for their subsequent biochemical and structural analysis. This p...
ORGANISM(S): Synechocystis Sp. Pcc 6803 (ncbitaxon:1148) 
2022-06-03 | MSV000089593 | MassIVE
TranscriptomicsTU-tagging: cell type specific RNA isolation from intact complex tissues
We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues. This method is useful for isolating RNA from cell types that are difficult to i...
ORGANISM(S): Drosophila melanogaster 
2010-06-19 | E-GEOD-15445 | biostudies-arrayexpress
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