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MetabolomicsNPC1 regulates the distribution of phosphatidylinositol 4 kinases at Golgi and Lysosome membranes
Cholesterol and phosphoinositides (PI) are two critically important lipids that are found in cellular membranes and dysregulated in many disorders. Therefore, uncovering molecular pathways connecting these essential lipids may offer new therapeutic insights. We report that loss of function of lysoso...
2021-09-15 | MTBLS2612 | MetaboLights
TranscriptomicsIdentification of the mRNAs interacting with the Nup59 nucleoporin using RNA-IP and microarrays in budding yeast
To specifically explore such mRNA localization events, we immunopurified the entire Nuclear Pore Complex scaffold using Nup59 as a bait and systematically analyzed all co-isolating mRNAs using Agilent microarrays in the budding yeast Saccharomyces cerevisiae. A mock IP was performed as a control exp...
ORGANISM(S): Saccharomyces cerevisiae 
2021-03-31 | E-MTAB-9521 | biostudies-arrayexpress
TranscriptomicsChiP-chip profiling of Drosophila melanogaster cell lines to identify targets for nuclear pore proteins Nup153 and Megator.
ChIP-chip experiment for nuclear pore proteins Nup153 and Mtor in Drosophila S2 and Kc cells. This experiment is related to E-MEXP-2523.
ORGANISM(S): Drosophila melanogaster 
2010-02-12 | E-MEXP-2525 | biostudies-arrayexpress
TranscriptomicsEffect of NPAP1/C15orf2 overexpression on transcriptome of HEK293 cells
To gain insight into the function of Nuclear pore associated protein 1 (NPAP1, formerly C15orf2), we overexpressed NPAP1 in HEK293 cells. We detected no significant difference between NPAP1-expression of induced and uninduced cells in three technical replicates, exept for an approximately 10-fold in...
ORGANISM(S): Homo sapiens 
2012-06-17 | E-GEOD-36575 | biostudies-arrayexpress
TranscriptomicsThe nuclear pore-associated TREX-2 complex employs the Mediator Med31 submodule as a docking site to regulate gene expression
Nuclear pore complexes (NPCs) influence gene expression besides their established function in nuclear transport. The TREX-2 complex localizes to the NPC basket and affects gene-NPC interactions, transcription and mRNA export. How TREX-2 regulates the gene expression machinery is unknown. Here, we sh...
ORGANISM(S): Saccharomyces cerevisiae 
2015-08-27 | E-GEOD-67431 | biostudies-arrayexpress
ProteomicsPNET1 is an on-demand component of the nuclear pore complex gating cell proliferation rates
The nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the nucleocytoplasmic shuttling of macromolecules. Here, we report that PNET1, a transmembrane nucleoporin conserved between humans and plants, is a dynamic, adaptable NPC component, exclusively expressed...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-03-10 | PXD054475 | Pride
Arabidopsis thaliana Callus Nuclear pore complex 
TranscriptomicsChromatin immunoprecipitation to identify gene-nuclear pore association in wild type and THO mutants of yeast
This work aimed at analysing the genomic loci that are aberrantly docked to the nucleopore in mutants of the THO complex
ORGANISM(S): Saccharomyces cerevisiae 
2008-08-15 | E-TABM-535 | biostudies-arrayexpress
GenomicsGenome-wide maps of Nup98 binding in human embryonic stem cells, neural progenitors, neuron cells and lung fibroblasts
Recent evidence suggests that nucleoporins, well known components that control nucleo-cytoplasmic trafficking, have wide-ranging functions in developmental gene regulation that potentially extend beyond their role in nuclear transport. Whether the unexpected role of nuclear pore proteins in transcri...
ORGANISM(S): Homo sapiens 
2013-07-17 | E-GEOD-48996 | biostudies-arrayexpress
TranscriptomicsGenome-wide microRNA analysis of NPC and non-NPC specimens gene expression
Analysis of differential expression between NPC and non-NPC tissues at miRNA expression level. We used a miRNA microarray platform to investigate the profile between NPC tissues and normal nasopharyngeal tissues to evaluate the relationship between miRNA expression and NPC. Recognition of the aberr...
ORGANISM(S): Homo sapiens 
2013-06-25 | E-GEOD-22587 | biostudies-arrayexpress
TranscriptomicsNuclear pore complex evolution: A trypanosome Mlp analog functions in chromosomal segregation but lacks transcriptional barrier activity
The nuclear pore complex (NPC) has dual roles in nucleocytoplasmic transport and chromatin organisation. In many eukaryotes the coiled coil Mlp/Tpr proteins of the NPC nuclear basket have specific roles in interactions with chromatin and defining specialised regions of active transcription, while Ml...
ORGANISM(S): Trypanosoma brucei 
2014-04-03 | E-GEOD-56436 | biostudies-arrayexpress
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