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GenomicsOff-Target Analysis of Zinc Finger Nucleases
Zinc Finger Nucleases (ZFNs) facilitate precise editing of DNA enabling targeted genomic modifications in vivo. ZFNs have been employed to obtain genetically modified plants and animals, and cell-based therapies utilizing ZFNs are undergoing clinical trials. However, many ZFNs display dose-dependent...
ORGANISM(S): Danio rerio 
2010-08-25 | E-GEOD-23762 | biostudies-arrayexpress
TranscriptomicsmicroRNA-like off-target transcript regulation by siRNAs
siRNAs mediate sequence-specific gene silencing in cultured mammalian cells but also silence unintended transcripts. Many siRNA off-target transcripts match the guide-strand ‘‘seed region,’’ similar to the way microRNAs match their target sites. The extent to which this seed-matched, microRNA-like, ...
ORGANISM(S): Mus musculus 
2009-01-31 | E-GEOD-14073 | biostudies-arrayexpress
TranscriptomicsWidespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity
Transfected siRNAs and miRNAs regulate numerous transcripts that have only limited complementarity to the active strand of the RNA duplex. This process reflects natural target regulation by miRNAs, but is an unintended (â??off-targetâ??) consequence of siRNA-mediated silencing. Here we demonstrate...
ORGANISM(S): Homo sapiens 
2006-09-12 | E-GEOD-5814 | biostudies-arrayexpress
GenomicsOff Target Effect Sequencing
CRISPR-CAS 9 gene knockout and off target effect sequencing
PRJEB12991 | ENA
TranscriptomicsOptimized siRNAs with a minimal off-target effect facilitate the screening of essential genes in cancer cells
siRNAs with dN are a promising and easy approach to characterize essential genes by minimizing the off-target effect in cancer cells. mRNA profiles of HCT 116 cells after the transfection of siRNAs or blank control by deep sequencing, using HiSeq 2000
ORGANISM(S): Homo sapiens 
2015-07-01 | E-GEOD-62968 | biostudies-arrayexpress
GenomicsOff-target effect of engineered AsCas12f variants
Off-target effect of engineered AsCas12f variants
PRJNA871035 | ENA
ProteomicsA Proteomic Platform to Identify Off-Target Proteins Associated with Therapeutic Modalities that Induce Protein Degradation or Gene Silencing
Novel modalities such as Proteolysis Targeting Chimera (PROTAC) and RNA interference (RNAi) have the ability to inadvertently alter the abundance of endogenous proteins. Currently available in vitro secondary pharmacology assays, which evaluate off-target binding or activity of small molecules, do ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD024312 | Pride
Proteomic platform for off-target screening for protac or rnai 
GenomicsmicroRNA-like off-target transcript regulation by siRNAs
microRNA-like off-target transcript regulation by siRNAs
PRJNA110373 | ENA
GenomicsIDLV capture for gRNA off-target analysis
IDLV capture for gRNA off-target analysis
PRJNA552824 | ENA
ProteomicsPhotoaffinity ligand of Cystic Fibrosis corrector VX-445 identifies SCCPDH as an off-target
Cystic fibrosis (CF) pharmacological correctors such as VX-445 are used in combinations as highly effective modulator therapy (HEMT) at CF clinics due to their efficacy in improving Cystic Fibrosis transmembrane conductance regulator (CFTR) protein trafficking and function. Yet, several side-effects...
ORGANISM(S): Homo sapiens (Human) 
2025-07-28 | PXD060898 | Pride
Elexacaftor Cystic fibrosis (cf) Vu439 Click chemistry Target identification Cystic fibrosis transmembrane conductance regulator (cftr) Off-target Mass spectrometry (ms) Proteomics Diazirine Metabolomics Chemoproteomics Corrector Photoaffinity ligand (pal) Vx-445 Saccharopine dehydrogenase-like oxidoreductase (sccpdh) 
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