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ProteomicsPhosphorylation site identifcaiton of OsHSFA4d
To identify the residues of OsHSFA4d phosphorylated by OsCDPK24, the in vitro kinase assays were conducted by incubating recombinant MBP-HA-OsCDPK24 + GST-OsHSFA4d and MBP-HA + GST-OsHSFA4d. The reaction was terminated by adding SDS loading buffer to a 1×concentration, and the reaction products were...
ORGANISM(S): Oryza sativa (Rice) 
2025-06-09 | PXD064745 | Pride
Phosphorylation site  oshsfa4d 
GenomicsOryza sativa
DAPseq of OsHSFA4d (PRJCA038540)
PRJDB36482 | ENA
ProteomicsGST-OsHSFA4d pulldown of rice total proteins
For liquid GST pull-down tandem with LC-MS/MS analysis, the gels were cut and sent to Shanghai Bioprofile (Shanghai, China) for analysis.
ORGANISM(S): Oryza sativa (Rice) 
2025-06-09 | PXD064744 | Pride
Gst pulldown  oshsfa4d 
ProteomicsPhosphorylation site identifcaiton of OsALDH2B1
To identify the residues of OsALDH2B1 phosphorylated by OsSERL1, the in vitro kinase assays were conducted by incubating recombinant MBP-OsSERL1 + MBP-OsALDH2B1 and MBP + MBP-OsALDH2B1. The reaction was terminated by adding SDS loading buffer to a 1×concentration, and the reaction products were subj...
ORGANISM(S): Oryza sativa (Rice) 
2026-08-24 | PXD071077 | Pride
Phosphorylation site identification Phosphorylation Protein 
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