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ProteomicsFishing for newly synthesized proteins with phosphonate-handles
Bioorthogonal chemistry introduces affinity-labels into biomolecules with minimal disruption to the original system and is widely applicable in a range of contexts. In proteomics, immobilized metal affinity chromatography (IMAC) enables enrichment of phosphopeptides with extreme sensitivity and sele...
ORGANISM(S): Homo sapiens (Human) 
2020-07-28 | PXD018272 | Pride
Bioorthogonal Affinity enrichment Lc-ms Click chemistry 
ProteomicsSITE-SPECIFIC ACTIVITY-BASED PROTEIN PROFILING USING PHOSPHONATE HANDLES
Most drug molecules target proteins. Identification of the exact drug binding sites on these proteins is essential to understand and predict how drugs affect protein structure and function. To address this challenge, we developed a strategy that uses IMAC-enrichable phosphonate affinity tags, for ef...
ORGANISM(S): Homo sapiens (Human) 
2023-01-10 | PXD036569 | Pride
Chemical proteomics  activity-based protein profiling  site-specific  phosphonate affinity handles  fe(iii)-immobilized metal affinity chromatography. 
UnknownFishing for newly synthesized proteins with phosphonate-handles.
Not available
S-EPMC7320153 | biostudies-literature
ProteomicsSite-Specific Competitive Kinase Inhibitor Target Profiling Using Phosphonate Affinity Tags
Protein kinases are prime targets for drug development due to their involvement in various cancers. However, selective inhibition of kinases, while avoiding off-target effects remains a significant challenge for the development of protein kinase inhibitors. Activity-based protein profiling (ABPP), i...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD058749 | Pride
Chemical proteomics  activity-based protein profiling  site-specific  protein kinase inhibitor profiling  ephrin receptor inhibitor  nvp-bhg712 
UnknownSite-Specific Activity-Based Protein Profiling Using Phosphonate Handles.
Not available
S-EPMC9803953 | biostudies-literature
ProteomicsDual-Probe Activity-Based Protein Profiling Reveals Site-Specific Binding Dynamics of EGFR-Directed Drugs
Comparative, dose-dependent analysis of interactions between small molecule drugs and their targets, as well as off-targets, in complex proteomes is crucial for selecting optimal drug candidates. The affinity of small molecules for targeted proteins is largely dictated by interactions between amino ...
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD045864 | Pride
Chemical proteomics  activity-based protein profiling  site-specific  concentration-dependent analysis  dual-probe comparison. 
UnknownRepurposing Antiviral Drugs for Orthogonal RNA-Catalyzed Labeling of RNA.
Not available
S-EPMC7318677 | biostudies-literature
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