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ProteomicsNEK1 autophosphorylation is disrupted by amyotrophic lateral sclerosis-associated missense variants: activity biomarkers and structural insights
Rare variants in NEK1, encoding a serine/threonine kinase, are established contributors to amyotrophic lateral sclerosis (ALS), but the biochemical effects of ALS-associated NEK1 missense variants on kinase activity have not been directly investigated. This project maps the phosphorylation landscape...
ORGANISM(S): Homo sapiens (Human) 
2026-09-02 | PXD079815 | Pride
Nek1 kinase  autophosphorylation  amyotrophic lateral sclerosis  als  motor neuron disease  phosphoproteomics  phosphorylation  kinase activity  activation loop  pthr156  pser14  pser418  lc-ms/ms  hcd  ethcd  u-2 os cells  gfp immunoprecipitation  kinase-dead mutant  missense variants 
ProteomicsProAlanase is an effective alternative to trypsin for proteomics applications and disulfide bond mapping
Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of digestion solution. In this study we characterized ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on the ...
ORGANISM(S): Homo sapiens (Human) Loxodonta africana (African elephant) 
2020-10-19 | PXD019039 | Pride
Sequence coverage Proline-rich Disulfide mapping Low-ph Proteomics Phosphorylation De novo sequencing Disulfide bonds Proalanase 
ProteomicsSty1 kinase-dependent phosphosite mapping on Atf1
In the fission yeast Schizosaccharomyces pombe, heterochromatic silencing is quite stable at pericentromeres but unstable at the mating-type (mat) locus under chronic heat stress. We found that the compromised gene silencing at the mat locus at elevated temperature is linked to the phosphorylation s...
ORGANISM(S): Schizosaccharomyces pombe 
2024-02-15 | PXD048330 | Pride
Atf1，phosphorylation，sty1，fission yeast (schizosaccharomyces pombe) 
ProteomicsPmk1 kinase-dependent phosphosite mapping on Slp1
In late mitosis, the timely polyubiquitylation and subsequent degradation of securin and cyclin B by anaphase-promoting complex/cyclosome (APC/C) play a critical role for anaphase onset and chromosome segregation. Binding of Cdc20, the co-activator of the APC/C, is critical for activation of APC/C a...
ORGANISM(S): Schizosaccharomyces pombe 
2024-10-15 | PXD048800 | Pride
Slp1 Pmk1 Phosphorylation Fission yeast (schizosaccharomyces pombe) 
UnknownThe Minimum Protein Staple? - Towards 'bio'-Baldwin's rules via inter-phosphosite linking in the MEK1 activation loop.
Not available
S-EPMC10806838 | biostudies-literature
ProteomicsProAlanase is an effective alternative to trypsin for proteomics applications and disulfide bond mapping_part2
Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of digestion solution. In this study we characterized ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on the ...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD021191 | Pride
Sequence coverage Proline-rich Disulfide mapping Low-ph Proteomics Phosphorylation De novo sequencing Disulfide bonds Histone ptms Proalanase Histone variants 
UnknownProAlanase is an Effective Alternative to Trypsin for Proteomics Applications and Disulfide Bond Mapping.
Not available
S-EPMC7710147 | biostudies-literature
ProteomicsProAlanase is an effective alternative to trypsin for proteomics applications and disulfide bond mapping_part3
Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of the digestion solution. In this study we characterize ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on t...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD021703 | Pride
Sequence coverage Proline-rich Low-ph Histones Proteomics Phosphorylation De novo sequencing Disulfide bonds Proalanase 
UnknownPhosphosite mapping of P-type plasma membrane H+-ATPase in homologous and heterologous environments.
Not available
S-EPMC3281613 | biostudies-literature
ProteomicsIdentification and phosphorylation site mapping of a sperm specific PKA C subunit isoform from Mytilus galloprovincialis
This project investigates a sperm-specific isoform of the catalytic (C) subunit of cAMP-dependent protein kinase (PKA) in the marine mussel Mytilus galloprovincialis, an externally fertilizing invertebrate widely used as a model for gamete activation studies. A C-subunit isoform purified from male g...
ORGANISM(S): Crenomytilus 
2026-09-07 | PXD076788 | Pride
Camp-dependent protein kinase  pka  catalytic subunit  lc–ms/ms  phosphoproteomics  phosphorylation sites  tio₂ enrichment  mytilus galloprovincialis  sperm 
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