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ProteomicsProAlanase is an effective alternative to trypsin for proteomics applications and disulfide bond mapping_part3
Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of the digestion solution. In this study we characterize ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on t...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD021703 | Pride
Sequence coverage Proline-rich Low-ph Histones Proteomics Phosphorylation De novo sequencing Disulfide bonds Proalanase 
ProteomicsProAlanase is an effective alternative to trypsin for proteomics applications and disulfide bond mapping
Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of digestion solution. In this study we characterized ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on the ...
ORGANISM(S): Homo sapiens (Human) Loxodonta africana (African elephant) 
2020-10-19 | PXD019039 | Pride
Sequence coverage Proline-rich Disulfide mapping Low-ph Proteomics Phosphorylation De novo sequencing Disulfide bonds Proalanase 
ProteomicsProAlanase is an effective alternative to trypsin for proteomics applications and disulfide bond mapping_part2
Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of digestion solution. In this study we characterized ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on the ...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD021191 | Pride
Sequence coverage Proline-rich Disulfide mapping Low-ph Proteomics Phosphorylation De novo sequencing Disulfide bonds Histone ptms Proalanase Histone variants 
UnknownProAlanase is an Effective Alternative to Trypsin for Proteomics Applications and Disulfide Bond Mapping.
Not available
S-EPMC7710147 | biostudies-literature
UnknownSequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen.
Not available
S-EPMC11035369 | biostudies-literature
ProteomicsAncient palaeoimmunology: diving deeper with a two enzyme proteomics approach
This study investigates the efficacy of proteomic analysis of human remains to identify active Mycobacterium leprae infections in the past. Mycobacterial diseases, like leprosy, have plagued human populations for millennia. Thanks to effective treatment options, leprosy is not as widespread and dead...
ORGANISM(S): Homo sapiens (Human) 
2024-05-24 | PXD040419 | Pride
Ancient proteins Leprosy Immune response Paleoimmunoproteomics Lc-ms/ms 
ProteomicsProteomic Analysis of Methylglyoxal Modifications Reveals Susceptibility of Glycolytic Enzymes to Dicarbonyl Stress
Methylglyoxal (MGO) is a highly reactive cellular metabolite that glycates lysine and arginine residues to form post-translational modifications known as advanced glycation end products. Because of their low abundance and low stoichiometry, few studies have reported their occurrence and site-specifi...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD032812 | Pride
Glycation Post-translational modifications Methylglyoxal Lc-ms/ms 
ProteomicsA male Denisovan mandible from Pleistocene Taiwan
Denisovans are an extinct hominin group defined by ancient genomes of Middle to Late Pleistocene fossils from southern Siberia. Although genomic evidence suggests their widespread distribution throughout eastern Asia and possibly Oceania, so far only a few fossils from the Altai and Tibet are confid...
ORGANISM(S): Homininae Palaeoloxodon sp. Suidae 
2025-04-09 | PXD054412 | Pride
Enamel Dentin Bone Penghu Palaeoproteomics Denisovan Taiwan Lc-ms/ms 
ProteomicsConsecutive digestion of archaeological skeletal proteomes with multiple proteases
Ancient skeletal proteomes are increasingly utilised for phylogenetic and evolutionary analysis. These proteomes are, however, often small and with low sequence coverage. We expand on previous observations which have shown that parallel digestion of Pleistocene skeletal proteomes increases proteome ...
ORGANISM(S): Bos taurus (Bovine) Equus caballus (Horse) 
2024-03-06 | PXD047069 | Pride
Skeletal proteome Palaeoproteomics Archaeology Protease 
ProteomicsDetection of IGF2BP1 phosphorylation in vitro and in vivo via Pro-Alanase treatment
In this project we aim to identify phosphorylation sites of IGF2BP1 that have not been discovered yet in mass spec experiments due to insufficient coverage. To this end we used Pro-Alanase treatment that produced detectable peptides in regions that are not covered by commonly used approaches. IGF2BP...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-11-13 | PXD056497 | Pride
Tyrosine phosphorylation Pro-alanase Igf2bp1 
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