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TranscriptomicsPoly(A)-tail profiling reveals an embryonic switch in translational control
Poly(A) tails enhance the stability and translation of most eukaryotic messenger RNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding of poly(A)-tail function. Here we describe poly(A)-tail length profiling by sequencing (PAL-seq) and apply it to measu...
ORGANISM(S): Arabidopsis thaliana 
2014-01-29 | E-GEOD-52809 | biostudies-arrayexpress
TranscriptomicsmRNA Poly(A)-tail Changes Specified by Deadenylation Broadly Reshape Translation in Drosophila Oocytes and Early Embryos
Because maturing oocytes and early embryos lack transcription, posttranscriptional regulatory processes must control their development. To better understand this control, we profiled translational efficiencies and poly(A)-tail lengths throughout Drosophila oocyte maturation and early embryonic deve...
ORGANISM(S): Drosophila melanogaster 
2016-08-08 | E-GEOD-83616 | biostudies-arrayexpress
TranscriptomicsPoly(A) tail length profiling of mRNAs from fission yeast
To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarr...
ORGANISM(S): Schizosaccharomyces pombe 
2007-05-16 | E-TABM-217 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of PAPS1-dependent polyadenylation identifies novel roles for functionally specialized poly(A) polymerases in Arabidopsis thaliana
The poly(A) tail at 3' ends of eukaryotic mRNAs promotes their nuclear export, stability and translational efficiency, and changes in its length can strongly impact gene expression. The Arabidopsis thaliana genome encodes three canonical nuclear poly(A) polymerases, PAPS1, PAPS2 and PAPS4. As shown ...
ORGANISM(S): Arabidopsis thaliana 
2015-07-30 | E-GEOD-57690 | biostudies-arrayexpress
TranscriptomicsSaccharomyces cerevisiae 3' poly(A) site mapping
The use of alternative polyadenylation sites is common and affects the post-transcriptional fate of mRNA, including its stability, localization, and translation. Here we present a method for genome-wide and strand-specific mapping of poly(A) sites and quantification of RNA levels at unprecedented ef...
ORGANISM(S): Saccharomyces cerevisiae 
2013-01-10 | E-GEOD-40110 | biostudies-arrayexpress
TranscriptomicsmTAIL-seq reveals dynamic poly(A) tail regulation in oocyte-to-embryo development
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Drosophila melanogaster 
2016-08-01 | E-GEOD-83732 | biostudies-arrayexpress
TranscriptomicsPoly(A) tail length change of miRNA targets in HeLa cells
MicroRNA induces deadenylation of its targets according to the current model in the scientific community, but this model is based on the studies of a few individual genes. We tested the model by examining the global effect of miRNA on poly(A) tail of the targets. Synthetic miR-1 mimic was transfecte...
ORGANISM(S): Homo sapiens 
2014-02-28 | E-GEOD-54114 | biostudies-arrayexpress
TranscriptomicsmTAIL-seq reveals dynamic poly(A) tail regulation in oocyte-to-embryo development [RNA-seq and mTAIL-seq Human and Drosphila]
Eukaryotic mRNAs are subject to multiple types of tailing which critically influence mRNA stability and translatability. To investigate RNA tails at the genomic scale, we previously developed TAIL-seq, but its low sensitivity precluded its application to biological materials of minute quantity. In t...
ORGANISM(S): Drosophila melanogaster 
2016-08-01 | E-GEOD-83731 | biostudies-arrayexpress
GenomicsPoly(A) Tail Length of L4 C. elegans
Poly(A) Tail Length of L4 C. elegans
PRJNA412860 | ENA
TranscriptomicsCleavage Factor Im as a key regulator of 3’ UTR length
In eukaryotes, the 3' ends of RNA polymerase II-generated transcripts are made in the majority of cases by site-specific endonucleolytic cleavage, followed by the addition of a poly(A) tail. By alternative polyadenylation, a gene can give rise to multiple mRNA isoforms that differ in the length of t...
ORGANISM(S): Homo sapiens 
2012-10-30 | E-GEOD-40137 | biostudies-arrayexpress
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