Manipulating protein synthesis is commonly applied in bio-related research to uncover protein functions and cellular activities. Multiple inhibitors with distinct mechanisms are widely used, but it is extraordinarily challenging to systematically determine and evaluate their inhibition efficiencies,...
The enzymatic activity of PADI4 was investigated by overexpression and chemical inhibition to determine the effects of arginine citrullination on histone displacement, reprogramming efficiency and the maintenance of pluripotent stem cells.
The antitumor drug daunorubicin exerts some of its cytotoxic effects by binding to DNA and inhibiting the transcription of different genes. We analysed this effect in vivo transcriptome level using the budding yeast Saccharomyces cerevisiae as a model and sublethal concentrations of the drug to min...
Inhibition of the nonsense mediated decay (NMD) mechanism in cells results in stabilization of transcripts carrying premature translation termination codons. A strategy referred to as gene indentification by NMD inhibition (GINI) has been proposed to identify genes carrying nonsense mutations (Noen...
African swine fever virus (ASFV) is an infectious disease with a mortality rate of nearly 100% in pigs; however, no safe commercial vaccines or antiviral drugs are currently available, which seriously threatens the global pig industry. Therefore, effective biologicals against ASFV are urgently ne...
In multiple myeloma, the objective is to assess the impact of syntenin knockout on the secretory profile of bone marrow stromal cells using the HS-5 model.
Human induced pluripotent stem cells (iPS cells) resemble embryonic stem cells and can differentiate into cell derivatives of all three germ layers. However, frequently the differentiation efficiency of iPS cells into some lineages is rather poor. Here, we found that fusion of iPS cells with human h...
To elucidate the genome-wide role of Ca2+/ calmodulin inhibition of E2A in regulation of BCR activation, we performed DNA microarray analysis of activated splenic B cells expressing wildtype or CaM-resistant E12 mutant m8N47 with and without anti-IgM treatment for 3 hour.The expression of a remarkab...
