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ProteomicsRevealing Corynebacterium glutamicum proteoforms through top-down proteomics
Corynebacterium glutamicum is a bacterium widely employed in the industrial production of amino acids as well as a broad range of other biotechnological products. The present study describes the characterization of C. glutamicum proteoforms, and their post-translational modifications (PTMs) employin...
ORGANISM(S): Corynebacterium glutamicum ATCC 13032 
2022-12-10 | PXD038038 | Pride
Top-down proteomics Corynebacterium glutamicum Proteoforms 
ProteomicsTop-down proteomics of membrane proteoforms from HeLa cells and mouse hippocampus
Comprehensive characterization of membrane proteins at the level of proteoforms in complex biological samples by top-down mass spectrometry is of vital importance in revealing their precise functions. Based on a novel membrane protein separation technology, we obtained a dataset of membrane proteofo...
ORGANISM(S): Homo Sapiens Mus Musculus 
2023-02-08 | PXD039949 |
ProteomicsDiversity of Amyloid-beta Proteoforms in the Alzheimer's Disease Brain
Amyloid-beta (Aβ) plays a key role in the pathogenesis of Alzheimer’s disease (AD), but little is known about the proteoforms present in human AD brain. We used high-resolution mass spectrometry to analyze intact, undigested Aβ from purified soluble aggregates and insoluble material in brains of 6 c...
ORGANISM(S): Homo sapiens (Human) 
2017-08-28 | PXD006552 | Pride
Post-translational modifications (ptms) Amyloid-beta Proteoforms Alzheimer’s disease Top-down mass spectrometry Truncations 
ProteomicsTissue expression of N-terminal proteoforms.
To evaluate the expression of N-terminal proteoforms (protein isoforms with different N-terminus expressed from the same gene) in healthy human tissues, we re-analyzed public proteomics data of the draft map of the human proteome developed by the Pandey group (PRIDE project PXD000561). Using ionbot ...
ORGANISM(S): Homo sapiens (Human) 
2023-06-12 | PXD039339 | Pride
Proteoforms Healthy tissue Human 
ProteomicsExtraordinary Diversity of Amyloid-beta Proteoforms in Human Alzheimer's Disease Brain
Amyloid-beta (Aβ) plays a key role in the neuropathogenesis of Alzheimer’s disease, but little is known about the proteoforms (i.e., all protein variants of a single gene including post-translational modifications and sequence variants) present in human AD brain. We used high-resolution mass spectro...
ORGANISM(S): Homo sapiens (Human) 
2017-09-04 | PXD005119 | Pride
Human ad brain  nlc-ms/ms 
ProteomicsEvaluation of Protein Ion Relative Ratio Quantification in Top-down Electrospray Ionization–Mass Spectrometry Using Site-Specific Acetylated Recombinant Histone H3 Proteoforms
Electrospray ionization (ESI)-mass spectrometry (MS) provides a crucial platform for analyzing post-translationally modified proteins. With continuous advances in MS instruments and data analysis approaches, analyzing intact proteoforms using a “top-down” approach has become highly feasible. To quan...
ORGANISM(S): Homo sapiens (Human) 
2025-09-29 | PXD064777 | Pride
Proteoforms Top-down proteomics Histone h3 Quantitative proteomics Protein ion relative ratio 
ProteomicsDenaturing and native top-down venomics: Mapping proteoforms and protein complexes from king cobra venom
Characterizing whole proteins by top-down proteomics avoids a step of inference encountered in the dominant bottom-up methodology when peptides are assembled computationally into proteins for identification. The direct interrogation of whole proteins and protein complexes from the venom of Ophiophag...
ORGANISM(S): Ophiophagus hannah (King cobra) (Naja hannah) 
2016-05-18 | PXD003403 | Pride
King cobra venom Top-down proteomics Proteoforms Native mass spectrometry Protein complexes 
UnknownQuantifying Homologous Proteins and Proteoforms.
Not available
S-EPMC6317479 | biostudies-literature
ProteomicsQuantifying homologous proteins and proteoforms
Many proteoforms - arising from alternative splicing, post-translational modifications (PTMs), or paralogous genes - have distinct biological functions, such as histone PTM proteoforms. However, their quantification by existing bottom-up mass-spectrometry (MS) methods is undermined by peptide-specif...
ORGANISM(S): Saccharomycetales (ncbitaxon:4892) Homo Sapiens (ncbitaxon:9606) 
2017-12-22 | MSV000081857 | MassIVE
ProteomicsCapillary Zone Electrophoresis-High Field Asymmetric Ion Mobility Spectrometry-Tandem Mass Spectrometry for Top-down Characterization of Histone Proteoforms
Characterization of histone proteoforms with various post-translational modifications (PTMs) is critical for a better understanding of functions of histone proteoforms in epigenetic control of gene expression. Mass spectrometry (MS)-based top-down proteomics (TDP) is a valuable approach for delineat...
ORGANISM(S): Bos taurus (Bovine) 
2023-12-07 | PXD041357 | Pride
Histone Top-down proteomics High-field asymmetric waveform ion mobility spectrometry Post-translational modification Capillary zone electrophoresis 
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