The intracristal space (ICS) is a distinct subdomain of the mitochondrial intermembrane space (IMS) where oxidative phosphorylation occurs. This region supposedly controls the dynamic demands regulating mitochondrial respiration; however, its other molecular components remain largely unknown. To rem...
This proof-of-principle experiment was designed to demonstrate the feasibility of proximity labeling for RNAM-bM-^@M-^Sprotein interactions IPL-seq on 293T-Rex expressing MSA-SNRPN70 (sample) or NFH-SNRPN70 (control)
Activation of the NACHT, LRR family pyrin domain containing 3 (NLRP3) inflammasome complex is an essential innate immune signalling mechanism. To reveal how NLRP3 inflammasome assembly and activation are controlled, in particular by components of the ubiquitin system, proximity labelling, affinity p...
We introduce pupylation-based proximity labelling (PUP-IT) as a tool for protein interaction detection in plant biology. We show that PUP-IT readily confirmed and extended protein interactions for several known protein complexes, across different types of plant systems.
Small heat shock proteins are chaperones that are essential in preserving the integrity of the cellular proteome during stress conditions. The human genome encodes ten small heat shock proteins (HspB1-HspB10), which often form polydisperse multimeric functional structures. Uniquely, HspB2 and HspB3 ...
