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ProteomicsMARCH5 interactome detection using PUP-IT
MARCH5 (also referred to as MITOL) is known as a mitochondrial ubiquitin ligase. It regulates the mitochondria homeostasis by ubiquitinating several important mitochondrial fusion/fission regulators. In this study, we used a proximity labeling method PUP-IT to systematically identify potential MARC...
ORGANISM(S): Homo Sapiens 
PXD029348 |
UnknownPUP-IT2 as an alternative strategy for PUP-IT proximity labeling.
Not available
S-EPMC9558124 | biostudies-literature
ProteomicsPupylation-based proximity labelling reveals regulatory factors in cellulose biosynthesis in Arabidopsis
We introduce pupylation-based proximity labelling (PUP-IT) as a tool for protein interaction detection in plant biology. We show that PUP-IT readily confirmed and extended protein interactions for several known protein complexes, across different types of plant systems.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Nicotiana tabacum (Common tobacco) 
2025-01-10 | PXD048346 | Pride
Pup-it Proximity labelling 
ProteomicsPTPRR interactome detection using PUP-IT (pupylation-based interaction tagging) Mass Spectrometry
To further stably express PTPRR (WT)-PafA or PTPRR (DA)-PafA in iPUP OVCAR5 cell, we subcloned PTPRR-WT or DA, respectively, into the PafA-IRES-EGFP plasmid. Each plasmid was packed into a lentivirus and then transduced into iPUP OVACR5 cells for 48 h. GFP-positive cells were sorted by flow cytometr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-30 | PXD015352 | Pride
Lc-ms Ptprr 
ProteomicsPUP-IT proximity-labeling of LST8-1 and RAPTOR1 interactors (constitutive expression)
PUP-IT proximity labeling of Arabidopsis Target of Rapamycin Complex (TORC) subunits LST8-1 and RAPTOR1, compared against a GFP control, with constitutively expressed FLAG::PUP(E). The sample metadata, sequence database and fragpipe workflow are uploaded under the 'Metadata' tag.
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-11-25 | MSV000096513 | MassIVE
ProteomicsPUP-IT proximity-labeling of LST8-1 and RAPTOR1 interactors (inducible expression)
PUP-IT proximity labeling of Arabidopsis Target of Rapamycin Complex (TORC) subunits LST8-1 and RAPTOR1, compared against a GFP control, with beta-estradiol-inducible expression of FLAG::PUP(E). The sample metadata, sequence database and fragpipe workflow are uploaded under the 'Metadata' tag.
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2025-01-15 | MSV000096862 | MassIVE
ProteomicsPhosphoproteomics of RAPTOR1 and LST8-1 PUP-IT samples (inducible expression)
PUP-IT proximity labeling of Arabidopsis Target of Rapamycin Complex (TORC) subunits LST8-1 and RAPTOR1, compared against a GFP control, with beta-estradiol-inducible expression of FLAG::PUP(E). The FLAG-enriched samples were additionally enriched for phosphorylated peptides. The sample metadata, se...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2025-01-16 | MSV000096866 | MassIVE
ProteomicsPUP-IT proximity-labeling of Arabidopsis TOR via yeast FKBP (constitutive expression)
PUP-IT proximity labeling of Arabidopsis Target of Rapamycin Complex (TORC), using the yeast FKBP protein (P20081) as a bait with and without rapamycin treatment and constitutive expression of FLAG::PUP(E). The sample metadata, sequence database and fragpipe workflow are uploaded under the 'Metadata...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2025-01-16 | MSV000096864 | MassIVE
ProteomicsPUP-IT proximity-labeling of LST8-1 and RAPTOR1 interactors (transient expression in N. benthamiana)
PUP-IT proximity labeling of Arabidopsis Target of Rapamycin Complex (TORC) subunits LST8-1 and RAPTOR1, compared against a GFP control, via transient expression in Nicotiana benthamiana. The sample metadata, sequence database and FragPipe workflow are uploaded under the 'Metadata' tag. A short R sc...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) Nicotiana Benthamiana (ncbitaxon:4100) 
2024-12-13 | MSV000096657 | MassIVE
ProteomicsSide-by-side comparison of AP-MS, Tri-functional affinity probe, BioID, PUP-IT and SPIDER to identify CheZ-interacting proteins
Comparison of CheZ interactors’ ratio of two replicate experiments using five different methods, i.e., Affinity-Purification, Tri-functional affinity probe Sulfo-SBED and proximity labeling methods BioID, SPIDER, PUP-IT
ORGANISM(S): Escherichia Coli 
2022-11-03 | PXD037952 |
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