Sort   by:  
 Page size 
TranscriptomicsRIP-chip analysis of the C. elegans GLD-2 and RNP-8 protein
C. elegans GLD-2 forms an active PAP with multiple RNA-binding partners to regulate diverse aspects of germline and early embryonic development. One GLD-2 partner, RNP-8, was previously shown to influence oocyte fate specification. To identify transcripts selectively associated with both GLD-2 and R...
ORGANISM(S): Caenorhabditis elegans 
2010-09-21 | E-GEOD-23843 | biostudies-arrayexpress
ProteomicsXL-MS of 5S RNP preribosomal complexes
The 5S RNP is assembled from its three components (5S rRNA, Rpl5/uL18, and Rpl11/uL5) before being incorporated into the pre-60S subunit. However, when ribosome synthesis is disturbed, a free 5S RNP can enter the MDM2–p53 pathway to regulate cell cycle and apoptotic signaling. Here we reconstitute a...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-06-13 | PXD040087 | Pride
5s rnp Cryo-em Mdm2 
ProteomicsirCLIP-RNP reveals patterns of dynamic protein associations on RNA
RNA binding proteins (RBPs) interact with RNA targets to control an array of processes, including RNA splicing, stability, transport, and translation1-3. Dysfunctional RNA-RBP interactions contribute to pathogenesis of a plethora of human diseases1,4,5, underscoring the need for a greater understand...
ORGANISM(S): Homo sapiens (Human) 
2025-01-28 | PXD053494 | Pride
Mass spectrometry Ms Crisslinking immunoprecipitation Rna binding proteins Clip Rbp 
TranscriptomicsHITS-CLIP of cytoplasmic SLBP RNP
UV cross-linking and immunoprecipiatation with high throughput sequencing of cytoplasmic SLBP RNP Polyclonal α-SLBP antibody was used to immunoprecipitate UV-crosslinked SLBP RNP from cytoplasmic HeLa lysates. Mock immunoprecipitation were also performed and serve as a negative control.
ORGANISM(S): Homo sapiens 
2015-12-16 | E-GEOD-61958 | biostudies-arrayexpress
TranscriptomicsProteomic and transcriptomic analyses of Dhh1 complexes reveals mitochondrial RNP complex association with yeast P-bodies
P-bodies (PB) are cytoplasmic RNP complexes that aggregate into foci when cells are exposed to stress. While the core components and stress response of PB and related RNP granules are conserved, it remains unclear how and why cells assemble mRNP complexes into granule foci during stress. We use mas...
ORGANISM(S): Saccharomyces cerevisiae 
2015-09-16 | E-GEOD-65989 | biostudies-arrayexpress
TranscriptomicsRIP-seq of polyribosomal SLBP RNP
UV cross-linking and immunoprecipiatation with high throughput sequencing of cytoplasmic SLBP RNP Polyclonal α-SLBP antibody was used to immunoprecipitate SLBP RNP from polyribosomal fractions of HeLa S3 lysates. Mock immunoprecipitation were also performed and serve as a negative control.
ORGANISM(S): Homo sapiens 
2015-12-16 | E-GEOD-62076 | biostudies-arrayexpress
TranscriptomicsRNA clamping by Vasa assembles a piRNA Amplifier complex on transposon transcripts
Germline-specific Piwi-interacting RNAs (piRNAs) protect the genome against selfish genetic elements and are essential for fertility in animals. piRNAs targeting active transposons are amplified by a feed-forward loop known as the Ping-pong cycle, which links endonucleolytic slicing of target RNA...
ORGANISM(S): Bombyx mori 
2014-06-04 | E-GEOD-57420 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse polysome vs RNP fractions from testis obtained from prepuberttal and adult animals to investigate post transcriptional control mechanisms in gametes
Gametes rely heavily on post-transcriptional control mechanisms to regulate their differentiation. In eggs, the storage and selective temporal activation of maternal mRNAs is essential for normal development. In the male, transcription ceases during spermiogenesis necessitating the post-transcriptio...
ORGANISM(S): Mus musculus 
2007-11-23 | E-GEOD-4711 | biostudies-arrayexpress
GenomicsDetection of RNAs binding to Vg1 RNP
Detection of RNAs binding to Vg1 RNP
PRJNA647395 | ENA
TranscriptomicsCRISPR-Cas9 mediated editing of the CD46 gene in primary human B cells prior to their infection with Epstein-Barr virus (EBV) – analysis of CD46 transcripts after 24 hours
Adenoid B cells from anonymous human donors were isolated and nucleofected with RNP complexes targeting two loci within exon 2 of the CD46 gene, which encodes a cell surface protein. One hour after nucleofection, the same number of control cells (w/o-RNP) and CD46-RNP complex treated cells were infe...
ORGANISM(S): Homo sapiens 
2020-10-18 | E-MTAB-9531 | biostudies-arrayexpress
Sort   by:  
 Page size