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ProteomicsAPEX2 labeling of Sec bodies in S2 cells
APEX2 was tagged to Sec24AB protein in S2 cells. Biotinylation was triggeredin resting cells and amino acid starved cells for comparative analyses of Sec24AB interactome. Sec24AB is localised to Sec bodies during starvation, thereby allowing the identification of protein components in Sec bodies.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-05-10 | PXD031601 | Pride
Proximity labeling  biotinylation  apex2  mass spectrometry  sec bodies  granules 
ProteomicsProteomic analysis of koala (Phascolarctos cinereus) spermatozoa and prostatic bodies
The aims of this study were to investigate the proteome of koala (Phascolarctos cinereus) spermatozoa and that of the prostatic bodies with which they form intimate contact at the moment of ejaculation. For this purpose, spermatozoa and prostatic bodies were obtained from four adult male koala by el...
ORGANISM(S): Phascolarctos cinereus (Koala) 
2021-08-23 | PXD024250 | Pride
Spermatozoa Koala Prostatic bodies Proteomics 
UnknownStress-induced phase separation of ERES components into Sec bodies precedes ER exit inhibition in mammalian cells.
Not available
S-EPMC10112967 | biostudies-literature
ProteomicsIdentifying subcellular structure components in Escherichia Coli by crosslinking and SEC-MS
Cells are comprised of a broad spectrum of structures that compartmentalize biochemical and signaling mechanisms. These structures can be comprised of many biomolecules, but especially lipids, proteins, and nucleic acids. Options are limited to quantify or discover new subcellular structures. We exp...
ORGANISM(S): Escherichia coli 
2026-05-25 | PXD054099 | Pride
Size-exclusion chromatography and mass spectrometry 
ProteomicsThe proteome of Processing Bodies in Arabidopsis.
An Affinity Purification (AP) step coupled with Proximity-dependent biotin ligation (PDL) reveals the proteome of Processing Bodies (PBs) in Arabidopsis.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-01-17 | PXD037701 | Pride
Arabidopsis Lc-ms/ms Processing bodies Proteome 
ProteomicsProteome of Deciliated Tetrahymena thermophila "Bodies" Fractionated by IEX or SEC.
Tetrahymena thermophil SB715 were deciliated by pH shock, collected by centrifugation, washed once and flash frozen. Lysate was made by grinding in liquid nitrogen and resuspension in buffer containing 0.2% NP40 with pipetting. Lysate was clarified by sequential centrifugations including 1 hour 130...
ORGANISM(S): Tetrahymena Thermophila (ncbitaxon:5911) 
2023-04-07 | MSV000091661 | MassIVE
UnknownNovel Components of the Stress Assembly Sec Body Identified by Proximity Labeling.
Not available
S-EPMC10093351 | biostudies-literature
UnknownActivation of IRE1, PERK and salt-inducible kinases leads to Sec body formation in Drosophila S2 cells.
Not available
S-EPMC8445602 | biostudies-literature
UnknownThe conserved SNARE SEC-22 localizes to late endosomes and negatively regulates RNA interference in Caenorhabditis elegans.
Not available
S-EPMC5311485 | biostudies-literature
TranscriptomicsIdentifying chromatin associations with promyelocytic leukemia nuclear bodies using immunoTRAP
Studies using fluorescence in situ hybridization (FISH) have attempted to determine whether specific gene loci associate with promyelocytic leukemia nuclear bodies (PML NBs). Two drawbacks accompany this approach; the lack of spatial resolution inherent with the technique, and the a priori basis fo...
ORGANISM(S): Homo sapiens 
2013-03-28 | E-GEOD-40554 | biostudies-arrayexpress
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