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ProteomicsAn automated intact glycopeptide enrichment method for serum site-specific N-glycoproteomic analysis
We developed an automated glycopeptide enrichment method for the analysis of serum site-specific N-glycoproteome. This automated method allowed for processing one sample within 20 min. It showed higher enrichment specificity, more intact glycopeptide identifications, and better quantitative reproduc...
ORGANISM(S): Homo Sapiens (human) 
2021-04-06 | PXD025151 | JPOST Repository
ProteomicsAutomated N-glycopeptide identification in glycoproteomics
Recent advances in software-driven glycopeptide identification in LC-MS/MS-based N-glycoproteomics have facilitated biochemical studies reporting thousands of intact N-glycopeptides, i.e. N-glycan-conjugated peptides, but the automated identification process remains to be scrutinized. Herein, we exp...
ORGANISM(S): Homo sapiens (Human) 
2016-08-17 | PXD004243 | Pride
Basigin Byonic Glycoproteomics Glycopeptide N-glycosylation Lc-ms/ms 
ProteomicsN-glycopeptide Signatures in Liver Diseases
(1) A N-glycopeptide method based on 18O/16O C-terminal labelling to obtain comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC-O18 labelling) and HBV-related liver cirrhosis (LC-O16 labelling) (2) MRM verification of N-glycopeptide alterations
ORGANISM(S): Homo Sapiens 
2019-09-10 | PXD013586 |
ProteomicsSite-Specific Glycosylation Profiling of Protein Subunit and Inactivated Virus Vaccines
Many protein subunit vaccines and biologics contain glycosylated antigens and antibodies, yet quantitative frameworks for comparing glycosylation across lots, manufacturers, and production platforms remain limited. We performed site specific glycosylation LC-MS/MS analysis for intact N-linked glycop...
ORGANISM(S): Influenza A virus (A/Panama/2007/1999(H3N2)) Influenza A virus (A/Philippines/2/1982(H3N2)) Human alphaherpesvirus 3 Influenza A virus (A/New Caledonia/20/1999(H1N1)) Severe acute respiratory syndrome coronavirus 2 Influenza A virus (A/Shandong/9/1993(H3N2)) 
2026-07-21 | PXD074672 | Pride
Glycosylation Mass spectrometry Site-specific Vaccines Proteomics 
ProteomicsSugarQuant: a streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics
Site-specific regulation of protein N-glycosylation is essential in human cells. However, accurate quantification of glycosylation sites and their individual glycan moieties in a cell-wide manner is still technically challenging. Here, we introduce SugarQuant, an integrated mass spectrometry-based p...
ORGANISM(S): Homo sapiens (Human) 
2020-08-24 | PXD018349 | Pride
Glycosylation 
ProteomicsSite-specific N- and O-glycosylation Analysis of Human Plasma Fibronectin
Human plasma fibronectin is an adhesive protein that plays a crucial role in wound healing. Many studies had indicated that glycans may mediate the expression and functions of fibronectin, yet a comprehensive understanding of its glycosylation is still missing. Here, we performed a comprehensive N- ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD025886 | Pride
Mass spectrometer Glycosylation Stepped normalized collision energy Operator Fibronectin 
ProteomicsSite-specific N-glycoproteomics of aging mouse uterus tissues for benchmarking the StrucGAP analysis platform
This project presents the site-specific N-glycoproteomics dataset of aging mouse uterus tissues used to demonstrate and validate StrucGAP, a modular and scalable downstream analysis platform for glycoproteomics. Uterine samples were collected from 2- and 12-month-old C57BL/6 female mice (n = 15 per ...
ORGANISM(S): Mus Musculus 
2025-04-23 | PXD063260 |
ProteomicsIn-depth site-specific O-glycosylation analysis of glycoproteins and endogenous peptides in cerebrospinal fluid (CSF) from healthy individual mild cognitive impairment (MCI) and Alzheimer disease (AD) patients
Site-specific O-glycoproteome mapping in complex biological system provides molecular basis for understanding the structure-function relationships of glycoproteins and their roles in physiological and pathological processes. Previous O-glycoproteome analysis in cerebrospinal fluid (CSF) focused on s...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-04-05 | MSV000087160 | MassIVE
ProteomicspGlyco3: Intact N- and O-glycopeptide search engine
Analyses of N- and O-glycopeptide using pGlyco3 as well as Byonic, MSFragger and MetaMorpheus. N-glycopeptides and O-Man glycopeptides of fission yeast and budding yeast are also analyzed using pGlyco3.
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) Homo Sapiens (ncbitaxon:9606) Schizosaccharomyces Pombe (ncbitaxon:4896) 
2021-01-27 | MSV000086771 | MassIVE
UnknownExploring site-specific N-glycosylation microheterogeneity of haptoglobin using glycopeptide CID tandem mass spectra and glycan database search.
Not available
S-EPMC3777712 | biostudies-literature
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