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Existing protocols for full-length single-cell RNA sequencing (scRNA-seq) produce libraries of high complexity (thousands of distinct genes) with outstanding sensitivity and specificity of transcript quantification. These full-length libraries have the advantage of allowing probing of transcript iso...
ORGANISM(S): Mus musculus 
Transcription by RNA polymerase II (RNAPII) is coupled to mRNA processing and chromatin modifications via the C-terminal domain (CTD) of its largest subunit, consisting of multiple repeats of the heptapeptide YSPTSPS. Pioneering studies showed that CTD serines are differentially phosphorylated along...
ORGANISM(S): Saccharomyces cerevisiae 
Purpose: To reveal the effects of thrombin on the proliferation, morphology, and gene expression in chondrocytes. Methods: Rat primary chondrocytes were cultured with RS, RP and RPT for 24hours, and RNA was extracted for sequencing. The chondrocytes’ morphology was investigated under inverted micros...
ORGANISM(S): Rattus norvegicus 
2024-09-08 | GSE184026 | GEO
The production of Tobacco Acid Pyrophosphatase (TAP), an enzyme commonly used for the removal of the 5â??cap of eukaryotic mRNAs, has been recently discontinued. Here we performed a comparison of current alternatives for the mapping of 5â??cap mRNAs and the associated transcription start sites in Sa...
ORGANISM(S): Saccharomyces cerevisiae 
Envenomation by urticating caterpillars of the family Megalopygidae is an underestimated public health issue in South America, with Podalia orsilochus being the main species implicated in cases reported in northeastern Argentina. This study aimed to characterize the toxicological properties of its v...
ORGANISM(S): Megalopygidae sp. BOLD:AAA3544 
2026-03-30 | PXD074932 | Pride
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