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The rat pheochromocytoma cell line PC12 cells were cultured in complete DMEM till 80% confluence, then placed at 5000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 ...
ORGANISM(S): Rattus norvegicus 
Investigation of whole genome expression pattern of 60 and 72 hours post fertilization Danio Rerio embryos exposed to TMT and vehicle control Embryos were exposed to 10uM TMT or control from 48hpf to 60 or 72 hpf. Three replicates were collected for each time point. 40 embryos were pooled to compris...
ORGANISM(S): Danio rerio 
Haloferax volcanii strain:TMT | isolate:cell culture Genome sequencing
This dataset contains an evaluation of TMT-based multiplexed proteomics on the Orbitrap Astral mass spectrometer.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-12-17 | MSV000096674 | MassIVE
This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex and TMT 6-plex reagents.
ORGANISM(S): Phaeosphaeria Nodorum Sn15 (ncbitaxon:321614) 
2016-03-17 | MSV000079594 | MassIVE
Low cost transcriptomic mapping of TMT associated neurotoxicity using human minibrains
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-01-29 | MSV000084859 | MassIVE
LC-MS-based profiling of proteomes with isobaric tag labeling from low-quantity biological and clinical samples, including needle-core biopsies and laser capture microdissection, has been challenging due to limited amount and sample loss during preparation. To address this problem, we developed OnM ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-18 | MSV000091734 | MassIVE
We used whole mouse genome microarrays to the effects of γ-TmT on global gene expression in the prostate TRAMP and age-matched C57BL/6 mice were administered either 600 mg/kg of γ-TmT or a control vehicle via oral gavage. Twelve hours after dosing, prostate tissues were collected for RNA extracti...
ORGANISM(S): Mus musculus 
Investigation of whole genome expression pattern of 60 and 72 hours post fertilization Danio Rerio embryos exposed to TMT and vehicle control
ORGANISM(S): Danio rerio 
2011-03-24 | GSE28131 | GEO
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